Figure 3.

Pharmacological inhibition of MLKs reduces FRA-1 protein expression in TNBC lines. (a) SUM-159 cells and (b) 4T1 cells were treated with vehicle, 400 nm CEP-1347 or 400 nm URMC-099 for 24 h. Cellular lysates were subjected to immunoblotting with indicated antibodies. Western blot quantification of the indicated protein normalized to actin is expressed as mean±s.d. from at least three independent experiments. (c) SUM-159 cells and (d) 4T1 cells were seeded on coverslips, treated±400 nm CEP-1347 for 24 h, and subjected to immunofluorescence staining against FRA-1 antibody (green). Nuclei were counterstained with DAPI (blue); Scale bar, 25 μm in c and 50 μm in d.