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. 2017 Jun 5;6(6):e344. doi: 10.1038/oncsis.2017.47

Figure 3.

Figure 3

Changes in endosomal and lysosomal morphology upon flotillin-1 knockdown. Immunofluorescent labelling for (a) LAMP3 or (b) LAMP1 in control siRNA and flotillin-1-knockdown HeLa cells. Quantification of the stainings for (c) LAMP3 and (d) LAMP1 show a significantly increased staining intensity for both endosomal markers in flotillin-1-knockdown cells. (e) Flotillin-1-knockout cells (F1-KO) show a similar phenotype with intense LAMP3 and LAMP1 staining that can be rescued upon expression of flotillin-1-EGFP (cells marked with *, images for GFP in Supplementary Figure S1g). (f) Quantification of the LAMP3 staining in e. The data in c, d, f are shown as mean±s.d. Cells from three independent experiments were evaluated (control siRNA cells: n=136, F1-siRNAa: n=135 and F1-siRNAb: n=149; control Hela: n=149, flotillin-1 KO: n=211, rescue cells: n=147). Statistical analysis in c, d was performed with Student’s t-test (two-tailed, unpaired), and in f with one-way analysis of variance (ANOVA) and Bonferroni post-test, in comparison with the control. ***P<0.001. Scale bar in a, b, e: 10 μm.