Figure 1.

Hepatic ACOT1 is increased during fasting. C57Bl/6J mice were either fed or fasted overnight (16 h). A: Acot1 expression was increased with fasting, as analyzed by RT-PCR (n = 5 mice). B: Western blotting (top) and densitometry (bottom) of ACOT1 in fed and fasted mice. C: Fixed liver sections from fed or fasted (16 h) mice were probed with the ACOT1 primary antibody and Alexa 488 secondary antibody. D: Western blots of nuclear preparations from fed and fasted mice. *P < 0.05. ATP-CL, ATP-citrate lyase.