Figure 4. DR4 is the direct target of miR-106b.

(A) Complementary pairing site between miR-106b and DR4 3′ UTR showed by TargetScan database. (B) Huh7 and HepG2 cells were co-transfected with miR-106b mimics (or miR-106b inhibitors) and luciferase reporters contained wildtype/mutant 3′-UTR of DR4. 48 h post transfection, luciferase activity was detected using Dual-Luciferase Reporter Assay System according to the manufacturer's instruction. *P < 0.05 vs. miR-control group.