Skip to main content
. 2017 May 11;8(26):43153–43168. doi: 10.18632/oncotarget.17820

Figure 3. IL-17RC silencing results in acquired-JNK activation but distinct c-Jun activities in B16 and 4T1 cells.

Figure 3

(a-d) IL-17RCKD and pSMP controls of B16 and 4T1 cells were treated with DMSO or one of the inhibitors for 48 hours. Cell proliferation was measured by MTT assay. All values are presented as the mean ± SEM of 4-6 replicates in two independent experiments. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; statistical analysis was compared with the DMSO control. ##p ≤ 0.01; ###p ≤ 0.001; statistical analysis was compared with the pSMP control. (e-h) Whole-cell extracts were harvested and immunoblotted to detect total or phosphorylated proteins as indicated. GAPDH was used as a loading control. Scanning densitometry of relative phospho (p)-JNK protein levels at 60 minutes post CM recovery was performed. All values are presented as the mean ± SEM of 4-6 replicates in two independent experiments; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; statistical analysis was compared with the pSMP control.