Table 2. List of identified mutations.

Nr.	Type	Location primary	NF1	RAS	BRAF	Other Mutations
1	M	G	E2174fs; L151fs
2	M	DM	R106*
3	M	G	R2258*	KRAS G12D
4	P	A	V1308fs			TERT S663N
5	P	G	G1425fs		V600E	TP53 Q165*
6	P	HN	H553fs			PIK3CA E109del
7	P	G	T889fs		N188S
8	M	A	R1306*
9	P	HN	T1184fs; D896N	KRAS G12A		ARID1A V700A; SF3B1 D894N
10	M	G	H55R
11	P	A	I183N			PTEN K163fs; SF3B1 R625H
12	P	D	M1376V		V600E	ARID1A R1202Q; ARID2 T1208A; MITF A401S
13	P	HN	V1308L	KRAS E63K		SF3B1 R625H
14	P	HN		KRAS G12F
15	P	HN		NRAS Q61R
16	P	HN		NRAS Q61R
17	P	HN		NRAS Q61K
18	P	HN		NRAS G13R		TERT Ser1104Thr
19	P	HN		NRAS Q61K		TERT P C228T; RAC1 N92K; GNA11 S267F;
20	U	G		NRAS Q61L		SF3B1 V634A
21	M	HN		NRAS A59D		TERT P C243T; TERT P C252T; SMARCA4 A152T
22	M	V		NRAS I46M
23	M	HN			V600E	TERT P C250T
24	P	HN			V600E	PIK3CA L896fs
25	P	A			V600K
26	M	HN				KIT L576P
27	M	Ur				TERT P C228T; WT1 D497N
28	M	G				GNAQ R183Q; MITF V487I
29	P	G				TP53 P58fs
30	P	G				ARID2 Y612C
31	M	HN				TERT L1002V
32	M	A				PTEN L108R
33	M	G				PIK3R1 T239M
34	P	G				KIT Y553del; MAP2K2 G286R
35	P	G				TERT R819H
36	P	HN				MITF N267K
37	P	A				TP53 C135R
38	R	HN				KIT V50L; PTEN C136R
39	P	G				TP53 P151A
40	U	G				ARID2 M545I; SF3B1 R625H
41	P	G				TERT R819H
42	U	A				MITF V487I; SMARCA4 R1260S
43	R	HN				SF3B1 T916S; CK4 R209C
44	P	HN				TP53 R175G
45	P	G				KIT, L783I
46	M	DM				KIT, I748T; BAP1, G579R: SF3B1, R625L
47	P	HN				BAP1 Y646C
48	P	HN				CTNNB1 Y331C
49	P	HN				TERT S953F
50	M	DM				CK4 V174M

Green, mutations known to be activating; red, loss of function mutations; black, missense mutation with unknown functional consequences. Abbreviations: Nr. sample number; M metastasis; P primary tumor; R recurrence; U unknown; fs frame shift; * = stop codon (nonsense mutation); HN Head and Neck, G Genital area, A Anorectum, D Digestive tract, Ur Urinary tract, DM data missing.

For more details including allele frequencies and cDNA annotations, see Supplementary Table 1.