Figure 4. Glibenclamide elevated membrane potential and subsequently increased reactive oxygen species.

ATP-sensitive potassium channel (K_ATP) is the target of glibenclamide and K_ATP consist of Kir6.1, Kir6.2, SUR1 and SUR2. The expression of Kir6.1 and Kir6.2 in hepatic tissue of mice (n=3) were tested by (A) immunohistochemical staining and (B) various tissues were detected by western blotting (n=3). 1 hour after treatment with glibenclamide (100μM) NCTC-1469 cells (C, E) were stained by DiBAC4(3) to test membrane potential (MP) and (D, F) were labeled by Reactive Oxygen Species Fluorogenic Probe to visualize ROS level. Simultaneously (G) the content of MDA was measured to indirectly demonstrate ROS level. (H) GSH concentration and (I) SOD activation were tested to reflect intracellular antioxidant ability. Data were presented as mean± SD. *P<0.05.