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. 2017 Mar 23;8(25):40568–40582. doi: 10.18632/oncotarget.16501

Figure 5. Glibenclamide activated Akt–NF-κB pathway via increased ROS.

Figure 5

NCTC-1469 cells were treated with glibenclamide (100μM) and then cells were harvested at 10min, 30min, 1 hour, 1.5 hour and 2 hour to extract proteins. (A, B, C) Expression of Akt and IκB were detected by western blotting and (D) translocation of p65 into cell nucleus was manifested by immunofluorescence. 1 hour after adding NAC (500μM) (E, F) the intracellular ROS level was marked by Reactive Oxygen Species Fluorogenic Probe and was visualized with confocal microscopy. (G, H) The expressions of Akt and IκB were detected by western blotting. n=3. Data were presented as mean± SD. *P<0.05 VS control group.