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. 2017 Mar 28;8(25):40741–40755. doi: 10.18632/oncotarget.16580

Figure 4. Heat stress activates cathepsin B release to cytosol in SW480 Cells.

Figure 4

SW480 cells underwent intense heat stress (43°C) for 2h and were further incubated at 37°C for different times as indicated (0h, 1h, 3h, 6h, 12h or 24h). A. Localization of cathepsin Bwas visualized by immunofluorescence using a confocal microscope (×200). B. Expression of cathepsin B was determined by Western blot. β-actin was run as an internal control. C. Quantification of Western blots for cathepsin B after heat stress. Graphs represent mean±SD of at least three independent experiments. *P < 0.05, compared with control group (37°C).