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. 2017 Apr 8;8(25):40115–40131. doi: 10.18632/oncotarget.16969

Figure 5. DLL4 and JAG1 induced endogenous JAG1 expression in tumour tissues.

Figure 5

A. Expression of JAG1 (red) and CD31 (green) as revealed by immunofluorescence double staining of tumour sections of U87. Magnification 200X. B. Endogenous hJAG1 expression in parental U87 cells (GFP-negative) sorted from co-culture of parental U87, U87-EV, U87-mDLL4 or U87-mJAG1 (GFP-positive) with an equal amount of parental U87 cells. hJAG1 expression in GFP-negative U87 cells was detected by FACS staining with anti-human JAG1 mAb (64D). ANOVA with Bonferroni's post-test. N = 3, Error bars represent SD. C. Endogenous hJAG1 expression in parental HMEC-1 cells (GFP-negative) sorted from co-culture of parental U87, U87-EV, U87-mDLL4 or U87-mJAG1 (GFP-positive) with an equal amount of parental HMEC-1 cells. hJAG1 expression in GFP-negative HMEC-1 cells was detected by FACS staining with anti-human JAG1 mAb (64D). ANOVA with Bonferroni's post-test. NS, no statistical difference. N = 3, Error bars represent SD. *P<0.05, ** P<0.01, *** P<0.001.