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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Virology. 2017 May 4;508:7–17. doi: 10.1016/j.virol.2017.04.029

Figure 3. PMA increases tax/rex mRNA stability in FS cells.

Figure 3

(A) RNA decay assay for RNA stability of different HTLV-1 transcripts upon PMA treatment. FS cells were treated with PMA for 15 min, washed, and followed by Act D treatment for 4 hours. RNAs were collected at the time indicated, and measured by RT-qPCR with specific primers for tax/rex, env, and gag/pol (full-length genome). Data are normalized to ASL, and presented as percentage mRNA remaining compared with time zero. Data shown are average of triplicates with standard deviation (error bars). PMA increases tax/rex mRNA stability, but does not change the stability of env or gag/pol mRNAs. (B) RNA decay assay for RNA stability of tax/rex mRNA upon vorinostat treatment. FS cells were treated with vorinostat for 4 hours, washed, and followed by Act D treatment for 4 hours. RNAs were collected at the times indicated, and analyzed as described in (A). Vorinostat does not change the stability of HTLV-1 tax/rex mRNA.