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. 2017 May 22;6(5):e339. doi: 10.1038/oncsis.2017.31

Figure 6.

Figure 6

DHEA attenuates TMZ-induced apoptosis. (a) After treatment with DHEA for 48 h, the cells were treated with TMZ for an additional 48 h in the absence of DHEA. The cells were collected for cell counting with a hemacytometer. (b) Cells pretreated with 1 μm of DHEA for 4 days were treated with TMZ for an additional 8 days. Crystal violet was used to stain the cells. Quantitative results for CYP17A1 (*P<0.05, **P<0.01 indicate the difference between the groups with or without TMZ treatment; #P<0.05 indicates the difference between TMZ-treated U373MG and DHEA+TMZ-treated U373MG cells) are shown in the right panel. (c) After treatment with DHEA for 48 h, the cells were treated with TMZ for an additional 48 h in the absence of DHEA. Protein lysates were subjected to western blotting with anti-PARP, anti-caspase 3 and anti-p53 antibodies.