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. 2017 May 15;6(5):e335. doi: 10.1038/oncsis.2017.37

Figure 6.

Figure 6

MiR-645 targets SOX30. (a) A schematic illustration of base-matching between miR-645 and the SOX30 3′-UTR (Upper panel) and the IFIT2 3′-UTR (Lower panel). Substitution of three consecutive bases (CUA to GAT) at the SOX30 or IFIT2 3′-UTR for mutant reporter constructs is also shown. (b) A schematic illustration of the luciferase reporter constructs. (c) The luciferase reporter activity of WiDr and EB cells transfected with the indicated reporter constructs was measured as mean±s.e., n=3. *P<0.05, Student's t-test. (d) Upper panel: WiDr and EB cells transduced with the miR-645 inhibitors or the control were introduced with the reporter constructs for 24 h. The luciferase reporter activity was measured. Lower panel: WiDr and EB cells transduced with the miR-645 inhibitors or the control were introduced with the reporter constructs for 24 h. The luciferase reporter activity was measured as mean±s.e., n=3. *P<0.05, Student's t-test. (e) Whole-cell lysates from WiDr and EB cells transduced with the miR-645 inhibitors or the control were subjected to Western blot analysis, n=3. (f) Western blot analysis of SOX30 in colon cancer samples #1–5 that expressed relatively low levels of miR-645 and #133–137 that expressed relatively high levels of miR-645 as shown in Figure 1c, n=3.