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. 2017 May 22;6(5):e338. doi: 10.1038/oncsis.2017.39

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Copyright © 2017 The Author(s)

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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TRPV4-specific activation of downstream signaling pathways. (a) Control and Trpv4 knocked-down cells were treated with DMSO or 4α-PDD and the lysates immunoblotted with the indicated antibodies. (b) The protein bands were measured and quantified using Image-J software. (c) 4T07 cells were either pre-treated or not with 10 μm ruthenium red for 1 h before stimulation with DMSO control or 4α-PDD. Lysates were then probed for TRPV4 and Actin control.