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. Author manuscript; available in PMC: 2017 Sep 6.
Published in final edited form as: Biochemistry. 2016 Aug 19;55(35):4885–4908. doi: 10.1021/acs.biochem.6b00293

Figure 7.

Figure 7

Kinetic stabilization effect on p53 by osmolyte or CDB3 on the kinetically controlled denaturation A) Wild type p53 was attached to BLI biosensors and subjected to a 5 min denaturation pulse. When 4 M glycerol was included in the denaturant pulse phase, the GroEL binding signal to the p53 loaded tip was significantly reduced. Glycerol was washed away prior to performing the GroEL binding phase. The data was load corrected to account for slight variations in p53 loading (> 4% standard deviation). B) CDB3 peptide at a final concentration of 200 μM was added both at an initial wash step after p53 was loaded onto the BLI biosensor and in the following denaturant pulse step. After the biosensor was removed from the denaturant solution and dipped into the loading buffer (to wash away denaturant and excess CDB3 peptide) for a 5 or 20-sec wash step, the biosensor was then immersed into the GroEL buffer containing 0.15 μM GroEL.