Figure 6. In vitro migration of astrocytes depends on Trpc3 and Trpc1.

(A) Representative images of scratched areas in cultured astrocytes isolated from wild-type (wt), Trpc3^−/− (Trpc3 ko), Trpc6^−/− (Trpc6 ko), Trpc3^−/−/c6^−/− (Trpc3/c6 dko), Trpc1^−/− (Trpc1 ko) and heterozygous TRPC3_T635A mutant (mwk) mice directly (0 h) and 4, 8 and 24 hours after scratching. (B) Data as in A, plotted as percentage of scratch area recovered by migrating astrocytes 4, 8 and 24 hours after scratching. Data in B represent means ± S.E.M. (n, number of experiments from x, number of cultures (n/x)). Asterisks assign the significant differences compared to the corresponding wt at the same hour (* P<0.05, ** P<0.01, *** P<0.001).