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. 1982;1(1):75–78. doi: 10.1002/j.1460-2075.1982.tb01127.x

Catalytic effects of elongation factor Ts on polypeptide synthesis

Tarmo Ruusala 1,*, Måns Ehrenberg 1, Charles G Kurland 1
PMCID: PMC552998  PMID: 16453409

Abstract

The kinetic parameters which characterize the interaction between elongation factor Tu (EF-Tu) and elongation factor Ts (EF-Ts) have been determined in a poly(uridylic acid)-primed translation system. The EF-Ts catalyzed release of GDP from EF-Tu was measured independently in a nucleotide exchange assay. We conclude that the rate-limiting step for the EF-Tu cycle in protein synthesis in the absence of EF-Ts is the release of GDP. By adding EF-Ts the time of this step is reduced from 90 s to 30 ms. Half maximal rate is obtained at an EF-Ts concentration of 2.5 x 10−6 M.

Keywords: EF-Ts, EF-Tu, kinetics, protein synthesis

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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