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. 1982;1(1):147–152. doi: 10.1002/j.1460-2075.1982.tb01138.x

Genetic Identification of functions of TL-DNA transcripts in octopine crown galls

J Leemans 1, R Deblaere 1, L Willmitzer 2, H De Greve 1, JP Hernalsteens 1, M Van Montagu 1,3, J Schell 2,3,*
PMCID: PMC553009  PMID: 16453404

Abstract

The TL-DNA in octopine crown galls encodes seven transcripts most, if not all, expressed from individual promoters. Site-specific deletions and substitutions in the T-region of the octopine plasmid pTiB6S3 indicate some of the functions of the TL-DNA transcripts. Two of the seven genes are sufficient to allow tumorous growth. T-DNA transfer and oncogenicity are controlled by different and independently acting functions. None of the transcripts of TL-DNA appear to be essential for T-DNA transfer. Four, possibly five, of the TL-DNA transcripts act by suppressing organ development. Shoot and root formation are suppressed by the action of different transcripts.

Keywords: Agrobacterium, Ti plasmids, site-specific mutagenesis, crown gall

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Selected References

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