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. 1982;1(2):173–179. doi: 10.1002/j.1460-2075.1982.tb01143.x

Nucleosome reconstitution on plasmid-inserted poly(dA) . poly(dT).

A Prunell
PMCID: PMC553016  PMID: 6325153

Abstract

Chromatin was reconstituted from core histones and recombinant plasmid DNAs carrying poly(dA) . poly(dT) inserts of various lengths. A 97-bp insert was found to occupy discrete and regularly-spaced positions on the edges of the nucleosome. This insert cannot, however, be entirely included due to a block in the center of the particle. In contrast, nucleosomes reconstitute on a shorter 20-bp insert. In this case, the insert shows a marked preference for the edges of the particle. Possible structural and physiological implications of these observations are discussed.

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