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. 1982;1(5):615–620. doi: 10.1002/j.1460-2075.1982.tb01217.x

Two main groups of mouse major urinary protein genes, both largely located on chromosome 4.

J O Bishop, A J Clark, P M Clissold, S Hainey, U Francke
PMCID: PMC553096  PMID: 6329695

Abstract

Fourteen different major urinary protein (MUP) genomic clones from BALB/c mice were isolated. By restriction site mapping, six of these form two sets of three overlapping clones. By the criterion of cross-hybridization, the 10 different genes fall into two groups of four (Group 1) and three (Group 2) genes, while three genes fall into neither group. Southern blot analysis of genomic DNA with Group 1 and Group 2 plasmid subclones shows that the haploid mouse (BALB/c) genome contains approximately 15 Group 1 genes, 12 Group 2 genes and at least seven MUP genes that belong to neither group. An analysis of mouse-Chinese hamster hybrid cell lines shows that most, if not all, Group 1 and Group 2 genes are located on mouse chromosome 4.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bennett K. L., Lalley P. A., Barth R. K., Hastie N. D. Mapping the structural genes coding for the major urinary proteins in the mouse: combined use of recombinant inbred strains and somatic cell hybrids. Proc Natl Acad Sci U S A. 1982 Feb;79(4):1220–1224. doi: 10.1073/pnas.79.4.1220. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bishop J. O., Davies J. A. Plasmid cloning vectors that can be nicked at a unique site. Mol Gen Genet. 1980;179(3):573–580. doi: 10.1007/BF00271747. [DOI] [PubMed] [Google Scholar]
  3. Bolivar F., Rodriguez R. L., Betlach M. C., Boyer H. W. Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9. Gene. 1977;2(2):75–93. doi: 10.1016/0378-1119(77)90074-9. [DOI] [PubMed] [Google Scholar]
  4. Clissold P. M., Bishop J. O. Molecular cloning of cDNA sequences transcribed from mouse liver endoplasmic reticulum poly(A)mRNA. Gene. 1981 Nov;15(2-3):225–235. doi: 10.1016/0378-1119(81)90132-3. [DOI] [PubMed] [Google Scholar]
  5. Davis M. M., Calame K., Early P. W., Livant D. L., Joho R., Weissman I. L., Hood L. An immunoglobulin heavy-chain gene is formed by at least two recombinational events. Nature. 1980 Feb 21;283(5749):733–739. doi: 10.1038/283733a0. [DOI] [PubMed] [Google Scholar]
  6. Efstratiadis A., Posakony J. W., Maniatis T., Lawn R. M., O'Connell C., Spritz R. A., DeRiel J. K., Forget B. G., Weissman S. M., Slightom J. L. The structure and evolution of the human beta-globin gene family. Cell. 1980 Oct;21(3):653–668. doi: 10.1016/0092-8674(80)90429-8. [DOI] [PubMed] [Google Scholar]
  7. FINLAYSON J. S., POTTER M., RUNNER C. R. ELECTROPHORETIC VARIATION AND SEX DIMORPHISM OF THE MAJOR URINARY PROTEIN COMPLEX IN INBRED MICE: A NEW GENETIC MARKER. J Natl Cancer Inst. 1963 Jul;31:91–107. [PubMed] [Google Scholar]
  8. Finlayson J. S., Asofsky R., Potter M., Runner C. C. Major urinary protein complex of normal mice: origin. Science. 1965 Aug 27;149(3687):981–982. doi: 10.1126/science.149.3687.981. [DOI] [PubMed] [Google Scholar]
  9. Finlayson J. S., Hudson D. M., Armstrong B. L. Location of the Mup-a locus on mouse linkage group 8. Genet Res. 1969 Dec;14(3):329–331. doi: 10.1017/s0016672300002159. [DOI] [PubMed] [Google Scholar]
  10. Finlayson J. S., Mushinski J. F., Hudson D. M., Potter M. Components of the major urinary protein complex in inbred mice: separation and peptide mapping. Biochem Genet. 1968 Sep;2(2):127–140. doi: 10.1007/BF01458712. [DOI] [PubMed] [Google Scholar]
  11. Francke U., Lalley P. A., Moss W., Ivy J., Minna J. D. Gene mapping in Mus musculus by interspecific cell hybridization: assignment of the genes for tripeptidase-1 to chromosome 10, dipeptidase-2 to chromosome 18, acid phosphatase-1 to chromosome 12, and adenylate kinase-1 to chromosome 2. Cytogenet Cell Genet. 1977;19(2-3):57–84. doi: 10.1159/000130799. [DOI] [PubMed] [Google Scholar]
  12. Francke U., Taggart R. T. Assignment of the gene for cytoplasmic superoxide dismutase (Sod-1) to a region of chromosome 16 and of Hprt to a region of the X chromosome in the mouse. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5230–5233. doi: 10.1073/pnas.76.10.5230. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hainey S., Bishop J. O. Allelic variation at several different genetic loci determines the major urinary protein phenotype of inbred mouse strains. Genet Res. 1982 Feb;39(1):31–39. doi: 10.1017/s0016672300020723. [DOI] [PubMed] [Google Scholar]
  14. Hudson D. M., Finlayson J. S., Potter M. Linkage of one component of the major urinary protein complex of mice to the brown coat color locus. Genet Res. 1967 Oct;10(2):195–198. doi: 10.1017/s0016672300010922. [DOI] [PubMed] [Google Scholar]
  15. Jovin T. M., Englund P. T., Bertsch L. L. Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase. J Biol Chem. 1969 Jun 10;244(11):2996–3008. [PubMed] [Google Scholar]
  16. Kemp D. J., Cory S., Adams J. M. Cloned pairs of variable region genes for immunoglobulin heavy chains isolated from a clone library of the entire mouse genome. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4627–4631. doi: 10.1073/pnas.76.9.4627. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kurtz D. T. Rat alpha 2u globulin is encoded by a multigene family. J Mol Appl Genet. 1981;1(1):29–38. [PubMed] [Google Scholar]
  18. Laskey R. A., Mills A. D. Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film. FEBS Lett. 1977 Oct 15;82(2):314–316. doi: 10.1016/0014-5793(77)80609-1. [DOI] [PubMed] [Google Scholar]
  19. RUEMKE P., THUNG P. J. IMMUNOLOGICAL STUDIES ON THE SEX-DEPENDENT PREALBUMIN IN MOUSE URINE AND ON ITS OCCURRENCE IN THE SERUM. Acta Endocrinol (Copenh) 1964 Sep;47:156–164. doi: 10.1530/acta.0.0470156. [DOI] [PubMed] [Google Scholar]
  20. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  21. Szoka P. R., Gallagher J. F., Held W. A. In vitro synthesis and characterization of precursors to the mouse major urinary proteins. J Biol Chem. 1980 Feb 25;255(4):1367–1373. [PubMed] [Google Scholar]
  22. Szoka P. R., Paigen K. Genetic regulation of mup production in recombinant inbred mice. Genetics. 1979 Sep;93(1):173–181. doi: 10.1093/genetics/93.1.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Szoka P. R., Paigen K. Regulation of mouse major urinary protein production by the Mup-A gene. Genetics. 1978 Nov;90(3):597–612. doi: 10.1093/genetics/90.3.597. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Yang R., Lis J., Wu R. Elution of DNA from agarose gels after electrophoresis. Methods Enzymol. 1979;68:176–182. doi: 10.1016/0076-6879(79)68012-6. [DOI] [PubMed] [Google Scholar]
  26. de Martinville B., Wyman A. R., White R., Francke U. Assignment of first random restriction fragment length polymorphism (RFLP) locus ((D14S1) to a region of human chromosome 14. Am J Hum Genet. 1982 Mar;34(2):216–226. [PMC free article] [PubMed] [Google Scholar]

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