Abstract
A highly specific antiserum was prepared against purified cauliflower mosaic virus viroplasm-protein (VmP). A virus specific in vitro major translation product (TPmaj), encoded by the 19S poly(A)+ RNA fraction from cauliflower mosaic virus infected turnip leaves, was recognized by this antiserum. The N-terminal sequence of TPmaj corresponds to the sequence following the first in-phase initiation codon in gene VI of the cauliflower mosaic virus genome. Both VmP and TPmaj have blocked termini and probably start from the same AUG codon.
Keywords: cauliflower mosaic virus, viroplasm, gene, in vitro, translation, immunopreciptation
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- Bloemendal H. The vertebrate eye lens. Science. 1977 Jul 8;197(4299):127–138. doi: 10.1126/science.877544. [DOI] [PubMed] [Google Scholar]
- Burgess R. R., Jendrisak J. J. A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography. Biochemistry. 1975 Oct 21;14(21):4634–4638. doi: 10.1021/bi00692a011. [DOI] [PubMed] [Google Scholar]
- Franck A., Guilley H., Jonard G., Richards K., Hirth L. Nucleotide sequence of cauliflower mosaic virus DNA. Cell. 1980 Aug;21(1):285–294. doi: 10.1016/0092-8674(80)90136-1. [DOI] [PubMed] [Google Scholar]
- Hager D. A., Burgess R. R. Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes. Anal Biochem. 1980 Nov 15;109(1):76–86. doi: 10.1016/0003-2697(80)90013-5. [DOI] [PubMed] [Google Scholar]
- Hohn T., Richards K., Geneviève-Lebeurier Cauliflower mosaic virus on its way to becoming a useful plant vector. Curr Top Microbiol Immunol. 1982;96:194–236. [PubMed] [Google Scholar]
- Howell S. H., Hull R. Replication of cauliflower mosaic virus and transcription of its genome in turnip leaf protoplasts. Virology. 1978 May 15;86(2):468–481. doi: 10.1016/0042-6822(78)90086-7. [DOI] [PubMed] [Google Scholar]
- Kozak M. Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes. Nucleic Acids Res. 1981 Oct 24;9(20):5233–5252. doi: 10.1093/nar/9.20.5233. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Lebeurier G., Hirth L., Hohn B., Hohn T. In vivo recombination of cauliflower mosaic virus DNA. Proc Natl Acad Sci U S A. 1982 May;79(9):2932–2936. doi: 10.1073/pnas.79.9.2932. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lebeurier G., Whitechurch O., Lesot A., Hirth L. Physical map of DNA from a new cauliflower mosaic virus strain. Gene. 1978 Nov;4(3):213–226. doi: 10.1016/0378-1119(78)90019-7. [DOI] [PubMed] [Google Scholar]
- Martelli G. P., Castellano M. A. Light and electron microscopy of the intracellular inclusions of cauliflower mosaic virus. J Gen Virol. 1971 Oct;13(1):133–140. doi: 10.1099/0022-1317-13-1-133. [DOI] [PubMed] [Google Scholar]
- Mayo M. A., Robinson D. J., Pérombelon M. C. Some properties of a bacterial protease with a specific effect on the protein in tobacco rattle virus particles. J Gen Microbiol. 1974 Nov;85(1):121–129. doi: 10.1099/00221287-85-1-121. [DOI] [PubMed] [Google Scholar]
- Muller S., Himmelspach K., Van Regenmortel M. H. Immunochemical localization of the C-terminal hexapeptide of histone H3 at the surface of chromatin subunits. EMBO J. 1982;1(4):421–425. doi: 10.1002/j.1460-2075.1982.tb01185.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Palmiter R. D. Prevention of NH2-terminal acetylation of proteins synthesized in cell-free systems. J Biol Chem. 1977 Dec 25;252(24):8781–8783. [PubMed] [Google Scholar]
- Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]