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. 1982;1(9):1049–1054. doi: 10.1002/j.1460-2075.1982.tb01295.x

Tandem CRP binding sites in the deo operon of Escherichia coli K-12.

P Valentin-Hansen
PMCID: PMC553161  PMID: 6329724

Abstract

The locations of DNA binding by the cyclic AMP receptor protein (CRP) in the deo operon of Escherichia coli have been determined by the DNase I footprinting procedure. Two high affinity sites were found around positions -35 and -90, preceding the second deo promoter. In vitro data on induction of gene fusions that join different parts of the deoP -2 regulatory region to the lac genes suggest that: (1) both CRP binding sites are needed for high expression from the deoP -2 region; and (2) negative regulation by the cytR repressor is accomplished by preventing the cAMP-CRP complex from binding to the second target.

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Selected References

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