Skip to main content
PMC home page
The EMBO Journal logoLink to The EMBO Journal
. 1982;1(11):1329–1335. doi: 10.1002/j.1460-2075.1982.tb01318.x

Sequence analysis and comparison of cDNAs of the zein multigene family .

D E Geraghty, J Messing, I Rubenstein
PMCID: PMC553212  PMID: 6897917

Abstract

The nucleotide sequence of two zein cDNAs in hybrid plasmids A20 and B49 have been determined. The insert in A20 is 921 bp long including a 5' non-coding region of 60 nucleotides, preceded by what is believed to be an artifactual sequence of 41 nucleotides, and a 3' non-coding region of 87 nucleotides. The B49 insert is 467 bp long and includes approximately one-half the protein coding sequence as well as a 3' non-coding region of 97 nucleotides. These sequences have been compared with the previously published sequence of another zein clone, A30 . A20 and A30 , both encoding 19 000 mol. wt. zeins , have approximately 85% homology at the nucleotide level. The B49 sequence, corresponding to a 22 000 mol. wt. zein, has approximately 65% homology to either A20 or A30 . All three zeins share common features including nearly identical amino acid compositions. In addition, the tandem repeats of 20 amino acids first seen in A30 are also present in A20 and B49 .

Full text

PDF
1329

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benoist C., O'Hare K., Breathnach R., Chambon P. The ovalbumin gene-sequence of putative control regions. Nucleic Acids Res. 1980 Jan 11;8(1):127–142. doi: 10.1093/nar/8.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bernard O., Gough N. M. Nucleotide sequence of immunoglobulin heavy chain joining segments between translocated VH and mu constant regions genes. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3630–3634. doi: 10.1073/pnas.77.6.3630. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blobel G., Walter P., Chang C. N., Goldman B. M., Erickson A. H., Lingappa V. R. Translocation of proteins across membranes: the signal hypothesis and beyond. Symp Soc Exp Biol. 1979;33:9–36. [PubMed] [Google Scholar]
  4. Burr B., Burr F. A., Rubenstein I., Simon M. N. Purification and translation of zein messenger RNA from maize endosperm protein bodies. Proc Natl Acad Sci U S A. 1978 Feb;75(2):696–700. doi: 10.1073/pnas.75.2.696. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Efstratiadis A., Posakony J. W., Maniatis T., Lawn R. M., O'Connell C., Spritz R. A., DeRiel J. K., Forget B. G., Weissman S. M., Slightom J. L. The structure and evolution of the human beta-globin gene family. Cell. 1980 Oct;21(3):653–668. doi: 10.1016/0092-8674(80)90429-8. [DOI] [PubMed] [Google Scholar]
  6. Geraghty D., Peifer M. A., Rubenstein I., Messing J. The primary structure of a plant storage protein: zein. Nucleic Acids Res. 1981 Oct 10;9(19):5163–5174. doi: 10.1093/nar/9.19.5163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Grantham R., Gautier C., Gouy M., Jacobzone M., Mercier R. Codon catalog usage is a genome strategy modulated for gene expressivity. Nucleic Acids Res. 1981 Jan 10;9(1):r43–r74. doi: 10.1093/nar/9.1.213-b. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hagen G., Rubenstein I. Complex organization of zein genes in maize. Gene. 1981 Apr;13(3):239–249. doi: 10.1016/0378-1119(81)90029-9. [DOI] [PubMed] [Google Scholar]
  9. Hu N. T., Peifer M. A., Heidecker G., Messing J., Rubenstein I. Primary structure of a genomic zein sequence of maize. EMBO J. 1982;1(11):1337–1342. doi: 10.1002/j.1460-2075.1982.tb01319.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hyldig-Nielsen J. J., Jensen E. O., Paludan K., Wiborg O., Garrett R., Jørgensen P., Marcker K. A. The primary structures of two leghemoglobin genes from soybean. Nucleic Acids Res. 1982 Jan 22;10(2):689–701. doi: 10.1093/nar/10.2.689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ingolia T. D., Craig E. A. Primary sequence of the 5' flanking regions of the Drosophila heat shock genes in chromosome subdivision 67B. Nucleic Acids Res. 1981 Apr 10;9(7):1627–1642. doi: 10.1093/nar/9.7.1627. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Inouye M., Halegoua S. Secretion and membrane localization of proteins in Escherichia coli. CRC Crit Rev Biochem. 1980;7(4):339–371. doi: 10.3109/10409238009105465. [DOI] [PubMed] [Google Scholar]
  13. Kozak M. Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes. Nucleic Acids Res. 1981 Oct 24;9(20):5233–5252. doi: 10.1093/nar/9.20.5233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Larkins B. A., Hurkman W. J. Synthesis and deposition of zein in protein bodies of maize endosperm. Plant Physiol. 1978 Aug;62(2):256–263. doi: 10.1104/pp.62.2.256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Larson R., Messing J. Apple II software for M13 shotgun DNA sequencing. Nucleic Acids Res. 1982 Jan 11;10(1):39–49. doi: 10.1093/nar/10.1.39. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  17. Messing J., Crea R., Seeburg P. H. A system for shotgun DNA sequencing. Nucleic Acids Res. 1981 Jan 24;9(2):309–321. doi: 10.1093/nar/9.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Park W. D., Lewis E. D., Rubenstein I. Heterogeneity of zein mRNA and protein in maize. Plant Physiol. 1980 Jan;65(1):98–106. doi: 10.1104/pp.65.1.98. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Pedersen K., Bloom K. S., Anderson J. N., Glover D. V., Larkins B. A. Analysis of the complexity and frequency of zein genes in the maize genome. Biochemistry. 1980 Apr 15;19(8):1644–1650. doi: 10.1021/bi00549a019. [DOI] [PubMed] [Google Scholar]
  20. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Soave C., Reggiani R., Di Fonzo N., Salamini F. Clustering of genes for 20 kd zein subunits in the short arm of maize chromosome 7. Genetics. 1981 Feb;97(2):363–377. doi: 10.1093/genetics/97.2.363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Viotti A., Abildsten D., Pogna N., Sala E., Pirrotta V. Multiplicity and diversity of cloned zein cDNA sequences and their chromosomal localisation. EMBO J. 1982;1(1):53–58. doi: 10.1002/j.1460-2075.1982.tb01123.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Viotti A., Sala E., Marotta R., Alberi P., Balducci C., Soave C. Genes and mRNAs coding for zein polypeptides in Zea mays. Eur J Biochem. 1979 Dec;102(1):211–222. doi: 10.1111/j.1432-1033.1979.tb06282.x. [DOI] [PubMed] [Google Scholar]
  24. Weaver C. A., Gordon D. F., Kemper B. Introduction by molecular cloning of artifactual inverted sequences at the 5' terminus of the sense strand of bovine parathyroid hormone cDNA. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4073–4077. doi: 10.1073/pnas.78.7.4073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Yamada Y., Avvedimento V. E., Mudryj M., Ohkubo H., Vogeli G., Irani M., Pastan I., de Crombrugghe B. The collagen gene: evidence for its evolutinary assembly by amplification of a DNA segment containing an exon of 54 bp. Cell. 1980 Dec;22(3):887–892. doi: 10.1016/0092-8674(80)90565-6. [DOI] [PubMed] [Google Scholar]

Articles from The EMBO Journal are provided here courtesy of Nature Publishing Group

RESOURCES