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. Author manuscript; available in PMC: 2017 Nov 17.
Published in final edited form as: Nature. 2017 May 17;545(7655):491–494. doi: 10.1038/nature22372

Figure 1. uORFsTBF1-mediated translational and TBF1 promoter-mediated transcriptional regulation.

Figure 1

a, Schematics of WT uORFsTBF1 or mutant uorfsTBF1. b–d, LUC activity (b), GFPER fluorescence (c) and cell death induced by TBF1-YFP (d), representative of 6 images. e, Dual-luciferase system. f, Translational changes of the reporter to different treatments. Mean of the LUC/RLUC activity ratios normalized to Mock (n = 3). g, LUC/RLUC mRNA levels in (f). Mean ± s.d. of LUC/RLUC mRNA normalized to Mock (n = 6). h, Endogenous TBF1 mRNA levels (n = 3). UBQ5, internal control. Solid circles, individual biological replicates. See Extended Data Fig. 2.