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. 1982;1(6):701–703. doi: 10.1002/j.1460-2075.1982.tb01233.x

Enhanced mutagenesis parallels enhanced reactivation of herpes virus in a human cell line.

C D Lytle, D C Knott
PMCID: PMC553271  PMID: 6329698

Abstract

U.v. irradiation of human NB-E cells results in enhanced mutagenesis and enhanced reactivation of u.v.-irradiated H-1 virus grown in those cells ( Cornelis et al., 1982). This paper reports a similar study using herpes simplex virus (HSV) in NB-E cells. The mutation frequency of HSV (resistance of virus plaque formation to 40 micrograms/ml iododeoxycytidine ) increased approximately linearly with exposure of the virus to u.v. radiation. HSV grown in unirradiated cells gave a slope of 1.8 X 10(-5)m2/J, with 3.2 X 10(-5)m2/J for HSV grown in cells irradiated (3 J/m2) 24 h before infection. There was no evidence for mutagenesis of unirradiated virus by irradiated cells, as seen with H-1 virus. Enhanced reactivation of irradiated HSV in parallel cultures increased virus survival, manifested as a change in slope of the final component of the two-component survival curve from a D0 of 27 J/m2 in unirradiated cells to 45 J/m2 in irradiated cells. Thus, enhanced mutagenesis and enhanced reactivation occurred for irradiated HSV in NB-E cells. The difference in the enhanced mutagenesis of HSV (dependent on damaged DNA sites) and of H-1 virus (primarily independent of damaged DNA sites) is discussed in terms of differences in DNA polymerases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Coppey J., Menezes S. Enhanced reactivation of ultraviolet-damaged herpes virus in ultraviolet pretreated skin fibroblasts of cancer prone donors. Carcinogenesis. 1981;2(8):787–793. doi: 10.1093/carcin/2.8.787. [DOI] [PubMed] [Google Scholar]
  2. Cornelis J. J., Lupker J. H., Klein B., van der Eb A. J. The effect of cell irradiation on mutation in ultraviolet-irradiated and intact simian virus 40. Mutat Res. 1981 Jun;82(1):1–10. doi: 10.1016/0027-5107(81)90132-9. [DOI] [PubMed] [Google Scholar]
  3. Cornelis J. J., Su Z. Z., Rommelaere J. Direct and indirect effects of ultraviolet light on the mutagenesis of parvovirus H-1 in human cells. EMBO J. 1982;1(6):693–699. doi: 10.1002/j.1460-2075.1982.tb01232.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cornelis J. J., Su Z. Z., Ward D. C., Rommelaere J. Indirect induction of mutagenesis of intact parvovirus H-1 in mammalian cells treated with UV light or with UV-irradiated H-1 or simian virus 40. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4480–4484. doi: 10.1073/pnas.78.7.4480. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DasGupta U. B., Summers W. C. Ultraviolet reactivation of herpes simplex virus is mutagenic and inducible in mammlian cells. Proc Natl Acad Sci U S A. 1978 May;75(5):2378–2381. doi: 10.1073/pnas.75.5.2378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hall J. D., Almy R. E. Evidence for control of herpes simplex virus mutagenesis by the viral DNA polymerase. Virology. 1982 Jan 30;116(2):535–543. doi: 10.1016/0042-6822(82)90146-5. [DOI] [PubMed] [Google Scholar]
  7. Lytle C. D., Day R. S., 3rd, Hellman K. B., Bockstahler L. E. Infection of UV-irradiated xeroderma pigmentosum fibroblasts by herpes simplex virus: study of capacity and Weigle reactivation. Mutat Res. 1976 Sep;36(3):257–264. doi: 10.1016/0027-5107(76)90235-9. [DOI] [PubMed] [Google Scholar]
  8. Lytle C. D., Goddard J. G., Lin C. H. Repair and mutagenesis of herpes simplex virus in UV-irradiated monkey cells. Mutat Res. 1980 Apr;70(2):139–149. doi: 10.1016/0027-5107(80)90153-0. [DOI] [PubMed] [Google Scholar]
  9. Lytle C. D. Host-cell reactivation in mammalian cells. I. Survival of ultra-violet-irradiated herpes virus in different cell-lines. Int J Radiat Biol Relat Stud Phys Chem Med. 1971;19(4):329–337. doi: 10.1080/09553007114550451. [DOI] [PubMed] [Google Scholar]
  10. Lytle C. D., Nikaido O., Hitchins V. M., Jacobson E. D. Host cell reactivation by excision repair is error-free in human cells. Mutat Res. 1982 Jun;94(2):405–412. doi: 10.1016/0027-5107(82)90303-7. [DOI] [PubMed] [Google Scholar]
  11. Purifoy D. J., Powell K. L. Herpes simplex virus DNA polymerase as the site of phosphonoacetate sensitivity: temperature-sensitive mutants. J Virol. 1977 Nov;24(2):470–477. doi: 10.1128/jvi.24.2.470-477.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rhode S. L., 3rd Replication process of the parvovirus H-1. III. Factors affecting H-1 RF DNA synthesis. J Virol. 1974 Oct;14(4):791–801. doi: 10.1128/jvi.14.4.791-801.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sarasin A., Benoit A. Induction of an error-prone mode of DNA repair in UV-irradiated monkey kidney cells. Mutat Res. 1980 Mar;70(1):71–81. doi: 10.1016/0027-5107(80)90059-7. [DOI] [PubMed] [Google Scholar]

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