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Real-time CCPCR with H1N1 influenza virus RNA template, the detected fluorescent intensity is indicated as a function of the amplification time: (a) experiment with the single-end heating strategy, (b) experiment with the dual-end heating strategy, for both (a) and (b), the solid line, dotted line, and dashed line correspond, respectively, to three typical repeated tests with the same target sample concentration of 10 TCID50/ml.
