Real-time CCPCR with H1N1 influenza virus RNA template. The detected fluorescent intensity is indicated as a function of the amplification time: (a) experiment with diluted samples, the solid line, dotted line, short dashed line, dashed and dotted line, and long dashed line correspond, respectively, to samples with concentrations of 1000, 100, 10, 1.0 TCID50/ml and negative control (NC), (b) experiment with samples with one concentration (100 TCID50/ml), the solid line, dotted line, and dashed line correspond, respectively, to three repeated tests.