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. 1987 Jan;6(1):259–264. doi: 10.1002/j.1460-2075.1987.tb04748.x

The halo-opsin gene. I. Identification and isolation

P Hegemann 1, A Blanck 1, H Vogelsang-Wenke 1, F Lottspeich 1, D Oesterhelt 1
PMCID: PMC553386  PMID: 15981335

Abstract

Halorhodopsin (HR), the light-driven chloride pump in halobacteria, was purified in the denatured as well as in the native state and chemically cleaved into peptide fragments. Isolation of peptide and liquid phase sequencing yielded ∼20% of the halo-opsin (HO) structure in non-overlapping peptides. Chemically synthesied oligodeoxynucleotides corresponding to a peptide sequence obtained from both HR preparations were used to screen a cosmid gene bank of Halobacterium halobium strain L-33. A positive clone contained cosmid pAB H47 which by subcloning and nucleotide sequencing was shown to encode at least part of the HO gene.

Keywords: halorhodopsin, chloride pump, partial structure, gene isolation, halobacteria, archaebacteria

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Selected References

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