Skip to main content
NCBI home page
The EMBO Journal logoLink to The EMBO Journal
. 1987 Feb;6(2):369–373. doi: 10.1002/j.1460-2075.1987.tb04764.x

Orientation and molecular map position of the complement genes in the mouse MHC.

U Müller, D Stephan, P Philippsen, M Steinmetz
PMCID: PMC553405  PMID: 3034587

Abstract

Over the past few years six gene clusters have been isolated from the major histocompatibility complex (MHC) of the BALB/c mouse encompassing a total of 1600 kb of DNA and 48 genes. The molecular distances between these gene clusters and the orientation of four of the six clusters on chromosome 17 is not known. Here we use pulse-field gradient gels and Southern blot hybridization to establish large-scale genomic restriction maps covering several hundreds of kb surrounding the three gene clusters located in the K, I, S, and D regions of the MHC. Comparison of the maps orients the complement gene clusters in the S region with the 21-OHB gene pointing towards the K end and the C2 gene pointing towards the D end of the MHC. The distances between the E alpha and 21-OHB genes is 430 kb and between the C2 and TNF-alpha genes at least 420 kb.

Full text

PDF
369

Images in this article

371Fig. 2.
on p.371
372Fig. 3.
on p.372

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carle G. F., Olson M. V. Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis. Nucleic Acids Res. 1984 Jul 25;12(14):5647–5664. doi: 10.1093/nar/12.14.5647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chaplin D. D., Woods D. E., Whitehead A. S., Goldberger G., Colten H. R., Seidman J. G. Molecular map of the murine S region. Proc Natl Acad Sci U S A. 1983 Nov;80(22):6947–6951. doi: 10.1073/pnas.80.22.6947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Figueroa F., Klein D., Tewarson S., Klein J. Evidence for placing the Neu-1 locus within the mouse H-2 complex. J Immunol. 1982 Nov;129(5):2089–2093. [PubMed] [Google Scholar]
  4. Hardy D. A., Bell J. I., Long E. O., Lindsten T., McDevitt H. O. Mapping of the class II region of the human major histocompatibility complex by pulsed-field gel electrophoresis. Nature. 1986 Oct 2;323(6087):453–455. doi: 10.1038/323453a0. [DOI] [PubMed] [Google Scholar]
  5. Levi-Strauss M., Tosi M., Steinmetz M., Klein J., Meo T. Multiple duplications of complement C4 gene correlate with H-2-controlled testosterone-independent expression of its sex-limited isoform, C4-Slp. Proc Natl Acad Sci U S A. 1985 Mar;82(6):1746–1750. doi: 10.1073/pnas.82.6.1746. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Müller U., Jongeneel C. V., Nedospasov S. A., Lindahl K. F., Steinmetz M. Tumour necrosis factor and lymphotoxin genes map close to H-2D in the mouse major histocompatibility complex. Nature. 1987 Jan 15;325(6101):265–267. doi: 10.1038/325265a0. [DOI] [PubMed] [Google Scholar]
  7. Ragoussis J., van der Bliek A., Trowsdale J., Ziegler A. Mapping of HLA genes using pulsed-field gradient electrophoresis. FEBS Lett. 1986 Aug 11;204(1):1–4. doi: 10.1016/0014-5793(86)81376-x. [DOI] [PubMed] [Google Scholar]
  8. Robins D. M., Malissen M., Hood L., Ferreira A., Walthall D., Mitchell M. Multiple C4/Slp genes distinguished by expression after transfection. Mol Cell Biol. 1986 Jan;6(1):134–141. doi: 10.1128/mcb.6.1.134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rosa P. A., Sepich D. S., Shreffler D. C., Ogata R. T. Mice constitutive for sex-limited protein (SLP) expression contain multiple Slp gene sequences. J Immunol. 1985 Jul;135(1):627–631. [PubMed] [Google Scholar]
  10. Schwartz D. C., Cantor C. R. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell. 1984 May;37(1):67–75. doi: 10.1016/0092-8674(84)90301-5. [DOI] [PubMed] [Google Scholar]
  11. Steinmetz M., Malissen M., Hood L., Orn A., Maki R. A., Dastoornikoo G. R., Stephan D., Gibb E., Romaniuk R. Tracts of high or low sequence divergence in the mouse major histocompatibility complex. EMBO J. 1984 Dec 1;3(12):2995–3003. doi: 10.1002/j.1460-2075.1984.tb02246.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Steinmetz M., Minard K., Horvath S., McNicholas J., Srelinger J., Wake C., Long E., Mach B., Hood L. A molecular map of the immune response region from the major histocompatibility complex of the mouse. Nature. 1982 Nov 4;300(5887):35–42. doi: 10.1038/300035a0. [DOI] [PubMed] [Google Scholar]
  13. Steinmetz M., Stephan D., Fischer Lindahl K. Gene organization and recombinational hotspots in the murine major histocompatibility complex. Cell. 1986 Mar 28;44(6):895–904. doi: 10.1016/0092-8674(86)90012-7. [DOI] [PubMed] [Google Scholar]
  14. Stephan D., Sun H., Lindahl K. F., Meyer E., Hämmerling G., Hood L., Steinmetz M. Organization and evolution of D region class I genes in the mouse major histocompatibility complex. J Exp Med. 1986 May 1;163(5):1227–1244. doi: 10.1084/jem.163.5.1227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. TE GROEN L. J., OOSTERHAGEN P. H., GELDENHUYS F. G. Primêre karsinoom van die tuba uterina met 'n verslag van 'n geval pre-operatief gediagnoseer. S Afr Med J. 1955 Jan 8;29(2):37–40. [PubMed] [Google Scholar]
  16. Tosi M., Lévi-Strauss M., Duponchel C., Meo T. Sequence heterogeneity of murine complementary DNA clones related to the C4 and C4-Slp isoforms of the fourth complement component. Philos Trans R Soc Lond B Biol Sci. 1984 Sep 6;306(1129):389–394. doi: 10.1098/rstb.1984.0099. [DOI] [PubMed] [Google Scholar]
  17. Uematsu Y., Kiefer H., Schulze R., Fischer-Lindahl K., Steinmetz M. Molecular characterization of a meiotic recombinational hotspot enhancing homologous equal crossing-over. EMBO J. 1986 Sep;5(9):2123–2129. doi: 10.1002/j.1460-2075.1986.tb04475.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Weis J. H., Seidman J. G., Housman D. E., Nelson D. L. Eucaryotic chromosome transfer: production of a murine-specific cosmid library from a neor-linked fragment of murine chromosome 17. Mol Cell Biol. 1986 Feb;6(2):441–451. doi: 10.1128/mcb.6.2.441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. White P. C., Chaplin D. D., Weis J. H., Dupont B., New M. I., Seidman J. G. Two steroid 21-hydroxylase genes are located in the murine S region. 1984 Nov 29-Dec 5Nature. 312(5993):465–467. doi: 10.1038/312465a0. [DOI] [PubMed] [Google Scholar]
  20. White P. C., New M. I., Dupont B. Cloning and expression of cDNA encoding a bovine adrenal cytochrome P-450 specific for steroid 21-hydroxylation. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1986–1990. doi: 10.1073/pnas.81.7.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wilton A. N., Charlton B. Order of class III genes relative to HLA genes determined by the haplotype method. Immunogenetics. 1986;24(2):79–83. doi: 10.1007/BF00373113. [DOI] [PubMed] [Google Scholar]
  22. Womack J. E., David C. S. Mouse gene for neuraminidase activity (Neu-1) maps to the D end of H-2. Immunogenetics. 1982;16(2):177–180. doi: 10.1007/BF00364404. [DOI] [PubMed] [Google Scholar]

Articles from The EMBO Journal are provided here courtesy of Nature Publishing Group

RESOURCES