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. 1987 Mar;6(3):801–807. doi: 10.1002/j.1460-2075.1987.tb04822.x

Oocyte-specific transcription of fs(1)K10: a Drosophila gene affecting dorsal–ventral developmental polarity

M Haenlin 1, C Roos 1, A Cassab 1, E Mohier 1
PMCID: PMC553465  PMID: 16453754

Abstract

The expression of the fs(1)K10 gene is required in early oogenesis for the establishment of the dorsal–ventral polarity of the oocyte, and later in the embryo. P-element-mediated transformation shows that the K10 function is located within a fragment of DNA of 5 kb, which encodes four RNA species. A major transcript of 3.1 kb is likely to be responsible for the K10 function. It is abundant in ovaries and in early developing embryos. Thus its expression profile corresponds closely to that which could be anticipated from the biological characteristics of the mutation. In situ hybridization on ovary sections shows that the gene is not only specifically transcribed in the germ line (which is consistent with the germ-line dependence of the mutation), but that its expression is also cell-specific since it is apparently restricted to the oocyte.

Keywords: Drosophila, dorsal–ventral polarity, transcription, in situ hybridization

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson K. V., Nüsslein-Volhard C. Information for the dorsal--ventral pattern of the Drosophila embryo is stored as maternal mRNA. Nature. 1984 Sep 20;311(5983):223–227. doi: 10.1038/311223a0. [DOI] [PubMed] [Google Scholar]
  2. Bender W., Spierer P., Hogness D. S. Chromosomal walking and jumping to isolate DNA from the Ace and rosy loci and the bithorax complex in Drosophila melanogaster. J Mol Biol. 1983 Jul 25;168(1):17–33. doi: 10.1016/s0022-2836(83)80320-9. [DOI] [PubMed] [Google Scholar]
  3. Cox K. H., DeLeon D. V., Angerer L. M., Angerer R. C. Detection of mrnas in sea urchin embryos by in situ hybridization using asymmetric RNA probes. Dev Biol. 1984 Feb;101(2):485–502. doi: 10.1016/0012-1606(84)90162-3. [DOI] [PubMed] [Google Scholar]
  4. Fyrberg E. A., Mahaffey J. W., Bond B. J., Davidson N. Transcripts of the six Drosophila actin genes accumulate in a stage- and tissue-specific manner. Cell. 1983 May;33(1):115–123. doi: 10.1016/0092-8674(83)90340-9. [DOI] [PubMed] [Google Scholar]
  5. Haenlin M., Steller H., Pirrotta V., Mohier E. A 43 kilobase cosmid P transposon rescues the fs(1)K10 morphogenetic locus and three adjacent Drosophila developmental mutants. Cell. 1985 Apr;40(4):827–837. doi: 10.1016/0092-8674(85)90342-3. [DOI] [PubMed] [Google Scholar]
  6. Melton D. A. Injected anti-sense RNAs specifically block messenger RNA translation in vivo. Proc Natl Acad Sci U S A. 1985 Jan;82(1):144–148. doi: 10.1073/pnas.82.1.144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Melton D. A., Krieg P. A., Rebagliati M. R., Maniatis T., Zinn K., Green M. R. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 1984 Sep 25;12(18):7035–7056. doi: 10.1093/nar/12.18.7035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Perrimon N., Engstrom L., Mahowald A. P. Developmental genetics of the 2E-F region of the Drosophila X chromosome: a region rich in "developmentally important" genes. Genetics. 1984 Nov;108(3):559–572. doi: 10.1093/genetics/108.3.559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Poole S. J., Kauvar L. M., Drees B., Kornberg T. The engrailed locus of Drosophila: structural analysis of an embryonic transcript. Cell. 1985 Jan;40(1):37–43. doi: 10.1016/0092-8674(85)90306-x. [DOI] [PubMed] [Google Scholar]
  10. Rosenberg U. B., Preiss A., Seifert E., Jäckle H., Knipple D. C. Production of phenocopies by Krüppel antisense RNA injection into Drosophila embryos. Nature. 1985 Feb 21;313(6004):703–706. doi: 10.1038/313703a0. [DOI] [PubMed] [Google Scholar]
  11. Rubin G. M., Spradling A. C. Genetic transformation of Drosophila with transposable element vectors. Science. 1982 Oct 22;218(4570):348–353. doi: 10.1126/science.6289436. [DOI] [PubMed] [Google Scholar]
  12. Rubin G. M., Spradling A. C. Vectors for P element-mediated gene transfer in Drosophila. Nucleic Acids Res. 1983 Sep 24;11(18):6341–6351. doi: 10.1093/nar/11.18.6341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Santamaria P., Nüsslein-Volhard C. Partial rescue of dorsal, a maternal effect mutation affecting the dorso-ventral pattern of the Drosophila embryo, by the injection of wild-type cytoplasm. EMBO J. 1983;2(10):1695–1699. doi: 10.1002/j.1460-2075.1983.tb01644.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Scalenghe F., Turco E., Edström J. E., Pirrotta V., Melli M. Microdissection and cloning of DNA from a specific region of Drosophila melanogaster polytene chromosomes. Chromosoma. 1981;82(2):205–216. doi: 10.1007/BF00286105. [DOI] [PubMed] [Google Scholar]
  15. Spradling A. C., Rubin G. M. Transposition of cloned P elements into Drosophila germ line chromosomes. Science. 1982 Oct 22;218(4570):341–347. doi: 10.1126/science.6289435. [DOI] [PubMed] [Google Scholar]
  16. Steward R., Ambrosio L., Schedl P. Expression of the dorsal gene. Cold Spring Harb Symp Quant Biol. 1985;50:223–228. doi: 10.1101/sqb.1985.050.01.028. [DOI] [PubMed] [Google Scholar]
  17. Steward R., McNally F. J., Schedl P. Isolation of the dorsal locus of Drosophila. Nature. 1984 Sep 20;311(5983):262–265. doi: 10.1038/311262a0. [DOI] [PubMed] [Google Scholar]
  18. Wieschaus E., Szabad J. The development and function of the female germ line in Drosophila melanogaster: a cell lineage study. Dev Biol. 1979 Jan;68(1):29–46. doi: 10.1016/0012-1606(79)90241-0. [DOI] [PubMed] [Google Scholar]

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