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. 1987 Apr;6(4):1011–1016. doi: 10.1002/j.1460-2075.1987.tb04853.x

The ATP requiring step in assembly of M13 procoat protein into microsomes is related to preservation of transport competence of the precursor protein.

H Wiech, M Sagstetter, G Müller, R Zimmermann
PMCID: PMC553497  PMID: 3297670

Abstract

M13 procoat protein is processed to transmembrane coat protein by dog pancreas microsomes after completion of synthesis and in the absence of the signal recognition particle (SRP)/docking protein system. ATP is required for fast and efficient processing of procoat protein by microsomes in a reticulocyte lysate. Requirement for ATP is also observed in the absence of ribosomes or docking protein. This indicates the existence of a unique assembly pathway for procoat protein into microsomes which depends on ATP but does not depend on the SRP/docking protein and ribosome/ribosome receptor systems. We suggest that the ATP requirement is linked to a so far unknown component of the reticulocyte lysate, acting on transport competence of precursor proteins.

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