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. 1987 Jun;6(6):1775–1779. doi: 10.1002/j.1460-2075.1987.tb02430.x

The structure of the Ultrabithorax promoter of Drosophila melanogaster

Gena Saari 1, Mariann Bienz 1
PMCID: PMC553554  PMID: 16453777

Abstract

The sequence of 4118 nucleotides upstream of the putative initiator codon of the D. melanogaster Ultrabithorax protein was determined. The transcription initiation site for the corresponding mRNA was identified by S1 nuclease mapping and primed extension. It appears that all embryonic RNA products that encode the first protein exon are initiated approximately one kilobase upstream of the initiator methionine, suggesting a unique Ultrabithorax promoter. The unusually long mRNA leader is unspliced. Upstream of the initiator codon occur two additional methonine codons; the first one is followed by an open reading frame encoding a putative polypeptide of 69 amino acids. We discuss the role of the leader and 5' flanking sequences with respect to transcriptional and posttranscriptional control of Ultrabithorax gene expression.

Keywords: homeotic gene, Ultrabithorax, promoter sequence, leader peptide

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Selected References

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  1. Akam M. E., Martinez-Arias A. The distribution of Ultrabithorax transcripts in Drosophila embryos. EMBO J. 1985 Jul;4(7):1689–1700. doi: 10.1002/j.1460-2075.1985.tb03838.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Akam M. E. The location of Ultrabithorax transcripts in Drosophila tissue sections. EMBO J. 1983;2(11):2075–2084. doi: 10.1002/j.1460-2075.1983.tb01703.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Beachy P. A., Helfand S. L., Hogness D. S. Segmental distribution of bithorax complex proteins during Drosophila development. Nature. 1985 Feb 14;313(6003):545–551. doi: 10.1038/313545a0. [DOI] [PubMed] [Google Scholar]
  4. Bender W., Akam M., Karch F., Beachy P. A., Peifer M., Spierer P., Lewis E. B., Hogness D. S. Molecular Genetics of the Bithorax Complex in Drosophila melanogaster. Science. 1983 Jul 1;221(4605):23–29. doi: 10.1126/science.221.4605.23. [DOI] [PubMed] [Google Scholar]
  5. Bender W., Weiffenbach B., Karch F., Peifer M. Domains of cis-interaction in the bithorax complex. Cold Spring Harb Symp Quant Biol. 1985;50:173–180. doi: 10.1101/sqb.1985.050.01.023. [DOI] [PubMed] [Google Scholar]
  6. Casanova J., Sánchez-Herrero E., Morata G. Prothoracic transformation and functional structure of the Ultrabithorax gene of Drosophila. Cell. 1985 Sep;42(2):663–669. doi: 10.1016/0092-8674(85)90123-0. [DOI] [PubMed] [Google Scholar]
  7. Garabedian M. J., Shepherd B. M., Wensink P. C. A tissue-specific transcription enhancer from the Drosophila yolk protein 1 gene. Cell. 1986 Jun 20;45(6):859–867. doi: 10.1016/0092-8674(86)90560-x. [DOI] [PubMed] [Google Scholar]
  8. Hayes P. H., Sato T., Denell R. E. Homoeosis in Drosophila: the ultrabithorax larval syndrome. Proc Natl Acad Sci U S A. 1984 Jan;81(2):545–549. doi: 10.1073/pnas.81.2.545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hogness D. S., Lipshitz H. D., Beachy P. A., Peattie D. A., Saint R. B., Goldschmidt-Clermont M., Harte P. J., Gavis E. R., Helfand S. L. Regulation and products of the Ubx domain of the bithorax complex. Cold Spring Harb Symp Quant Biol. 1985;50:181–194. doi: 10.1101/sqb.1985.050.01.024. [DOI] [PubMed] [Google Scholar]
  10. Jones K. A., Yamamoto K. R., Tjian R. Two distinct transcription factors bind to the HSV thymidine kinase promoter in vitro. Cell. 1985 Sep;42(2):559–572. doi: 10.1016/0092-8674(85)90113-8. [DOI] [PubMed] [Google Scholar]
  11. Khalili K., Brady J., Khoury G. Translational regulation of SV40 early mRNA defines a new viral protein. Cell. 1987 Feb 27;48(4):639–645. doi: 10.1016/0092-8674(87)90242-x. [DOI] [PubMed] [Google Scholar]
  12. Kioussis D., Vanin E., deLange T., Flavell R. A., Grosveld F. G. Beta-globin gene inactivation by DNA translocation in gamma beta-thalassaemia. Nature. 1983 Dec 15;306(5944):662–666. doi: 10.1038/306662a0. [DOI] [PubMed] [Google Scholar]
  13. Kozak M. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell. 1986 Jan 31;44(2):283–292. doi: 10.1016/0092-8674(86)90762-2. [DOI] [PubMed] [Google Scholar]
  14. Laughon A., Scott M. P. Sequence of a Drosophila segmentation gene: protein structure homology with DNA-binding proteins. Nature. 1984 Jul 5;310(5972):25–31. doi: 10.1038/310025a0. [DOI] [PubMed] [Google Scholar]
  15. Lawrence P. A., Morata G. The elements of the bithorax complex. Cell. 1983 Dec;35(3 Pt 2):595–601. doi: 10.1016/0092-8674(83)90091-0. [DOI] [PubMed] [Google Scholar]
  16. Lewis E. B. A gene complex controlling segmentation in Drosophila. Nature. 1978 Dec 7;276(5688):565–570. doi: 10.1038/276565a0. [DOI] [PubMed] [Google Scholar]
  17. Martinez-Arias A., Lawrence P. A. Parasegments and compartments in the Drosophila embryo. Nature. 1985 Feb 21;313(6004):639–642. doi: 10.1038/313639a0. [DOI] [PubMed] [Google Scholar]
  18. Morata G., Kerridge S. Sequential functions of the bithorax complex of Drosophila. Nature. 1981 Apr 30;290(5809):778–781. doi: 10.1038/290778a0. [DOI] [PubMed] [Google Scholar]
  19. Mueller P. P., Hinnebusch A. G. Multiple upstream AUG codons mediate translational control of GCN4. Cell. 1986 Apr 25;45(2):201–207. doi: 10.1016/0092-8674(86)90384-3. [DOI] [PubMed] [Google Scholar]
  20. Parkhurst S. M., Corces V. G. Forked, gypsys, and suppressors in Drosophila. Cell. 1985 Jun;41(2):429–437. doi: 10.1016/s0092-8674(85)80016-7. [DOI] [PubMed] [Google Scholar]
  21. Pelham H. R. A regulatory upstream promoter element in the Drosophila hsp 70 heat-shock gene. Cell. 1982 Sep;30(2):517–528. doi: 10.1016/0092-8674(82)90249-5. [DOI] [PubMed] [Google Scholar]
  22. Proudfoot N. J. Transcriptional interference and termination between duplicated alpha-globin gene constructs suggests a novel mechanism for gene regulation. Nature. 1986 Aug 7;322(6079):562–565. doi: 10.1038/322562a0. [DOI] [PubMed] [Google Scholar]
  23. Regulski M., Harding K., Kostriken R., Karch F., Levine M., McGinnis W. Homeo box genes of the Antennapedia and bithorax complexes of Drosophila. Cell. 1985 Nov;43(1):71–80. doi: 10.1016/0092-8674(85)90013-3. [DOI] [PubMed] [Google Scholar]
  24. Riddihough G., Pelham H. R. Activation of the Drosophila hsp27 promoter by heat shock and by ecdysone involves independent and remote regulatory sequences. EMBO J. 1986 Jul;5(7):1653–1658. doi: 10.1002/j.1460-2075.1986.tb04408.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Staden R. Measurements of the effects that coding for a protein has on a DNA sequence and their use for finding genes. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):551–567. doi: 10.1093/nar/12.1part2.551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sánchez-Herrero E., Vernós I., Marco R., Morata G. Genetic organization of Drosophila bithorax complex. Nature. 1985 Jan 10;313(5998):108–113. doi: 10.1038/313108a0. [DOI] [PubMed] [Google Scholar]
  27. White R. A., Wilcox M. Distribution of Ultrabithorax proteins in Drosophila. EMBO J. 1985 Aug;4(8):2035–2043. doi: 10.1002/j.1460-2075.1985.tb03889.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. White R. A., Wilcox M. Protein products of the bithorax complex in Drosophila. Cell. 1984 Nov;39(1):163–171. doi: 10.1016/0092-8674(84)90202-2. [DOI] [PubMed] [Google Scholar]
  29. Wilde C. D., Akam M. Conserved sequence elements in the 5' region of the Ultrabithorax transcription unit. EMBO J. 1987 May;6(5):1393–1401. doi: 10.1002/j.1460-2075.1987.tb02380.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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