Skip to main content
PMC home page
The EMBO Journal logoLink to The EMBO Journal
. 1987 Jul;6(7):1967–1974. doi: 10.1002/j.1460-2075.1987.tb02459.x

The human homologue of the mouse t-complex gene, TCP1, is located on chromosome 6 but is not near the HLA region.

K Willison 1, A Kelly 1, K Dudley 1, P Goodfellow 1, N Spurr 1, V Groves 1, P Gorman 1, D Sheer 1, J Trowsdale 1
PMCID: PMC553584  PMID: 3653076

Abstract

Southern blot analysis indicates that there are four sequences in the human genome related to the mouse t-complex gene Tcp-1. All four genes were cloned and partial sequencing showed that one of them was a functional gene, and the other three were pseudogenes. The human sequences were all approximately 90% related to each other and 82-89% related to the mouse Tcp-1a sequence. Human TCP1 cDNA clones from both fibrosarcoma and B cell lines confirmed that there was a single expressed gene. mRNA transcripts of different sizes were accounted for by two different polyadenylation signals. The human TCP1 gene shared some amino acid substitutions with the mouse t-complex allele (Tcp-1a) which were not found in Tcp-1b. The functional human TCP1 gene was mapped, using a panel of somatic cell hybrids, as well as in situ analysis, to the long arm of chromosome 6 at 6q23-qter and thus is not closely linked to the HLA complex on the short arm. For this reason and others it is unlikely that there is a human equivalent of the mouse t-complex.

Full text

PDF
1967

Images in this article

1967Fig. 1.
on p.1967
1971Fig. 5.
on p.1971

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews P. W., Knowles B. B., Goodfellow P. N. A human cell-surface antigen defined by a monoclonal antibody and controlled by a gene on chromosome 12. Somatic Cell Genet. 1981 Jul;7(4):435–443. doi: 10.1007/BF01542988. [DOI] [PubMed] [Google Scholar]
  2. Awdeh Z. L., Raum D., Yunis E. J., Alper C. A. Extended HLA/complement allele haplotypes: evidence for T/t-like complex in man. Proc Natl Acad Sci U S A. 1983 Jan;80(1):259–263. doi: 10.1073/pnas.80.1.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bailey E. Segregation distortion within the equine MHC; analogy to a mouse T/t-complex trait. Immunogenetics. 1986;24(4):225–229. doi: 10.1007/BF00364526. [DOI] [PubMed] [Google Scholar]
  4. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  5. Croce C. M., Koprowski H. Somatic cell hybrids between mouse peritoneal macrophages and SV40-transformed human cells. I. Positive control of the transformed phenotype by the human chromosome 7 carrying the SV40 genome. J Exp Med. 1974 Nov 1;140(5):1221–1229. doi: 10.1084/jem.140.5.1221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dudley K., Potter J., Lyon M. F., Willison K. R. Analysis of male sterile mutations in the mouse using haploid stage expressed cDNA probes. Nucleic Acids Res. 1984 May 25;12(10):4281–4293. doi: 10.1093/nar/12.10.4281. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Edwards Y. H., Parkar M., Povey S., West L. F., Parrington J. M., Solomon E. Human myosin heavy chain genes assigned to chromosome 17 using a human cDNA clone as probe. Ann Hum Genet. 1985 May;49(Pt 2):101–109. doi: 10.1111/j.1469-1809.1985.tb01681.x. [DOI] [PubMed] [Google Scholar]
  8. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  9. Geurts van Kessel A. H., den Boer W. C., van Agthoven A. J., Hagemeijer A. Decreased tumorigenicity of rodent cells after fusion with leukocytes from normal and leukemic donors. Somatic Cell Genet. 1981 Nov;7(6):645–656. doi: 10.1007/BF01538754. [DOI] [PubMed] [Google Scholar]
  10. Goodfellow P. N., Banting G., Trowsdale J., Chambers S., Solomon E. Introduction of a human X-6 translocation chromosome into a mouse teratocarcinoma: investigation of control of HLA-A, B, C expression. Proc Natl Acad Sci U S A. 1982 Feb;79(4):1190–1194. doi: 10.1073/pnas.79.4.1190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Goodfellow P., Banting G., Levy R., Povey S., McMichael A. A human X-linked antigen defined by a monoclonal antibody. Somatic Cell Genet. 1980 Nov;6(6):777–787. doi: 10.1007/BF01538976. [DOI] [PubMed] [Google Scholar]
  12. Gubler U., Hoffman B. J. A simple and very efficient method for generating cDNA libraries. Gene. 1983 Nov;25(2-3):263–269. doi: 10.1016/0378-1119(83)90230-5. [DOI] [PubMed] [Google Scholar]
  13. Hall A., Brown R. Human N-ras: cDNA cloning and gene structure. Nucleic Acids Res. 1985 Jul 25;13(14):5255–5268. doi: 10.1093/nar/13.14.5255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Harper M. E., Franchini G., Love J., Simon M. I., Gallo R. C., Wong-Staal F. Chromosomal sublocalization of human c-myb and c-fes cellular onc genes. Nature. 1983 Jul 14;304(5922):169–171. doi: 10.1038/304169a0. [DOI] [PubMed] [Google Scholar]
  15. Harper M. E., Saunders G. F. Localization of single copy DNA sequences of G-banded human chromosomes by in situ hybridization. Chromosoma. 1981;83(3):431–439. doi: 10.1007/BF00327364. [DOI] [PubMed] [Google Scholar]
  16. Heisterkamp N., Groffen J., Stephenson J. R., Spurr N. K., Goodfellow P. N., Solomon E., Carritt B., Bodmer W. F. Chromosomal localization of human cellular homologues of two viral oncogenes. Nature. 1982 Oct 21;299(5885):747–749. doi: 10.1038/299747a0. [DOI] [PubMed] [Google Scholar]
  17. Herrmann B., Bućan M., Mains P. E., Frischauf A. M., Silver L. M., Lehrach H. Genetic analysis of the proximal portion of the mouse t complex: evidence for a second inversion within t haplotypes. Cell. 1986 Feb 14;44(3):469–476. doi: 10.1016/0092-8674(86)90468-x. [DOI] [PubMed] [Google Scholar]
  18. Heyes J., Austin P., Bodmer J., Bodmer W., Madrigal A., Mazzilli M. C., Trowsdale J. Monoclonal antibodies to HLA-DP-transfected mouse L cells. Proc Natl Acad Sci U S A. 1986 May;83(10):3417–3421. doi: 10.1073/pnas.83.10.3417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Hobart M. J., Rabbitts T. H., Goodfellow P. N., Solomon E., Chambers S., Spurr N., Povey S. Immunoglobulin heavy chain genes in humans are located on chromosome. Ann Hum Genet. 1981 Oct;45(Pt 4):331–335. doi: 10.1111/j.1469-1809.1981.tb00346.x. [DOI] [PubMed] [Google Scholar]
  20. Lader E., Clark B. T., Jhanwar S. C., Chaganti R. S., Bennett D. Definitive chromosomal location of the H-2 complex by in situ hybridization to pachytene chromosomes. Immunogenetics. 1985;22(1):49–54. doi: 10.1007/BF00430593. [DOI] [PubMed] [Google Scholar]
  21. Lee J. S., Trowsdale J., Bodmer W. F. cDNA clones coding for the heavy chain of human HLA-DR antigen. Proc Natl Acad Sci U S A. 1982 Jan;79(2):545–549. doi: 10.1073/pnas.79.2.545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Lyon M. F. Male sterility of the mouse t-complex is due to homozygosity of the distorter genes. Cell. 1986 Jan 31;44(2):357–363. doi: 10.1016/0092-8674(86)90770-1. [DOI] [PubMed] [Google Scholar]
  23. Lyon M. F. Transmission ratio distortion in mouse t-haplotypes is due to multiple distorter genes acting on a responder locus. Cell. 1984 Jun;37(2):621–628. doi: 10.1016/0092-8674(84)90393-3. [DOI] [PubMed] [Google Scholar]
  24. Lyon M. F., Zenthon J., Evans E. P., Burtenshaw M. D., Dudley K., Willison K. R. Location of the t complex on mouse chromosome 17 by in situ hybridization with Tcp-1. Immunogenetics. 1986;24(2):125–127. doi: 10.1007/BF00373121. [DOI] [PubMed] [Google Scholar]
  25. Meunier H. F., Carson S., Bodmer W. F., Trowsdale J. An isolated beta 1 exon next to the DR alpha gene in the HLA-D region. Immunogenetics. 1986;23(3):172–180. doi: 10.1007/BF00373818. [DOI] [PubMed] [Google Scholar]
  26. Nadeau J. H., Berger F. G., Kelley K. A., Pitha P. M., Sidman C. L., Worrall N. Rearrangement of genes located on homologous chromosomal segments in mouse and man: the location of genes for alpha- and beta-interferon, alpha-1 acid glycoprotein-1 and -2, and aminolevulinate dehydratase on mouse chromosome 4. Genetics. 1986 Dec;114(4):1239–1255. doi: 10.1093/genetics/114.4.1239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Nadeau J. H., Taylor B. A. Lengths of chromosomal segments conserved since divergence of man and mouse. Proc Natl Acad Sci U S A. 1984 Feb;81(3):814–818. doi: 10.1073/pnas.81.3.814. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Nagarajan L., Louie E., Tsujimoto Y., ar-Rushdi A., Huebner K., Croce C. M. Localization of the human pim oncogene (PIM) to a region of chromosome 6 involved in translocations in acute leukemias. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2556–2560. doi: 10.1073/pnas.83.8.2556. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Philipsen M., Kristensen B. Preliminary evidence of segregation distortion in the SLA system. Anim Blood Groups Biochem Genet. 1985;16(2):125–133. doi: 10.1111/j.1365-2052.1985.tb01460.x. [DOI] [PubMed] [Google Scholar]
  30. Quax-Jeuken Y., Quax W., van Rens G., Khan P. M., Bloemendal H. Complete structure of the alpha B-crystallin gene: conservation of the exon-intron distribution in the two nonlinked alpha-crystallin genes. Proc Natl Acad Sci U S A. 1985 Sep;82(17):5819–5823. doi: 10.1073/pnas.82.17.5819. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Rogers J. H. The origin and evolution of retroposons. Int Rev Cytol. 1985;93:187–279. doi: 10.1016/s0074-7696(08)61375-3. [DOI] [PubMed] [Google Scholar]
  32. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Shin H. S., Flaherty L., Artzt K., Bennett D., Ravetch J. Inversion in the H-2 complex of t-haplotypes in mice. Nature. 1983 Nov 24;306(5941):380–383. doi: 10.1038/306380a0. [DOI] [PubMed] [Google Scholar]
  34. Silver L. M., Artzt K., Bennett D. A major testicular cell protein specified by a mouse T/t complex gene. Cell. 1979 Jun;17(2):275–284. doi: 10.1016/0092-8674(79)90153-3. [DOI] [PubMed] [Google Scholar]
  35. Silver L. M., Remis D. Five of the nine genetically defined regions of mouse t haplotypes are involved in transmission ratio distortion. Genet Res. 1987 Feb;49(1):51–56. doi: 10.1017/s0016672300026720. [DOI] [PubMed] [Google Scholar]
  36. Silver L. M., Uman J., Danska J., Garrels J. I. A diversified set of testicular cell proteins specified by genes within the mouse t complex. Cell. 1983 Nov;35(1):35–45. doi: 10.1016/0092-8674(83)90205-2. [DOI] [PubMed] [Google Scholar]
  37. Skow L. C., Donner M. E. The locus encoding alpha A-crystallin is closely linked to H-2K on mouse chromosome 17. Genetics. 1985 Aug;110(4):723–732. doi: 10.1093/genetics/110.4.723. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Solomon E., Bobrow M., Goodfellow P. N., Bodmer W. F., Swallow D. M., Povey S., Noël B. Human gene mapping using an X/autosome translocation. Somatic Cell Genet. 1976 Mar;2(2):125–140. doi: 10.1007/BF01542626. [DOI] [PubMed] [Google Scholar]
  39. Swallow D. M., Solomon E., Pajunen L. Immunochemical analysis of the N-acetyl hexosaminidases in human-mouse hybrids made using a double selective system. Cytogenet Cell Genet. 1977;18(3):136–148. doi: 10.1159/000130758. [DOI] [PubMed] [Google Scholar]
  40. Szabo P., Grzeschik K. H., Siniscalco M. A human autosomal phosphoglycerate kinase locus maps near the HLA cluster. Proc Natl Acad Sci U S A. 1984 May;81(10):3167–3169. doi: 10.1073/pnas.81.10.3167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Szymura J. M., Wabl M. R., Klein J. Mouse mitochondrial superoxide dismutase locus is on chromosome 17. Immunogenetics. 1981;14(3-4):231–240. doi: 10.1007/BF00342192. [DOI] [PubMed] [Google Scholar]
  42. Tani K., Singer-Sam J., Munns M., Yoshida A. Molecular cloning and structure of an autosomal processed gene for human phosphoglycerate kinase. Gene. 1985;35(1-2):11–18. doi: 10.1016/0378-1119(85)90152-0. [DOI] [PubMed] [Google Scholar]
  43. Viegas-Péquignot E., Dutrillaux B., Prod'Homme M., Petter F. Chromosomal phylogeny of Muridae: a study of 10 genera. Cytogenet Cell Genet. 1983;35(4):269–278. doi: 10.1159/000131879. [DOI] [PubMed] [Google Scholar]
  44. Weitkamp L. R. Linkage of GLO with HLA and Bf. Effect of population and sex on recombination frequency. Tissue Antigens. 1976 May;7(5):273–279. doi: 10.1111/j.1399-0039.1976.tb01068.x. [DOI] [PubMed] [Google Scholar]
  45. Willison K. R., Dudley K., Potter J. Molecular cloning and sequence analysis of a haploid expressed gene encoding t complex polypeptide 1. Cell. 1986 Mar 14;44(5):727–738. doi: 10.1016/0092-8674(86)90839-1. [DOI] [PubMed] [Google Scholar]
  46. Yunis J. J., Prakash O. The origin of man: a chromosomal pictorial legacy. Science. 1982 Mar 19;215(4539):1525–1530. doi: 10.1126/science.7063861. [DOI] [PubMed] [Google Scholar]

Articles from The EMBO Journal are provided here courtesy of Nature Publishing Group

RESOURCES