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. 1987 Jul;6(7):2003–2010. doi: 10.1002/j.1460-2075.1987.tb02464.x

Periodic DNA synthesis in cell-free extracts of Xenopus eggs.

C J Hutchison 1, R Cox 1, R S Drepaul 1, M Gomperts 1, C C Ford 1
PMCID: PMC553589  PMID: 3653080

Abstract

Cell-free extracts prepared from unfertilized eggs of Xenopus laevis support DNA synthesis on sperm pronuclei. Continuous labelling studies using [3H]dCTP and pulse labelling studies using [32P]dCTP demonstrate that synthesis occurs in short bursts of 40 min, which are punctuated by periods of 20-40 min during which no synthesis occurs. Density substitution experiments using bromodeoxyuridine demonstrate that this synthesis involves the initiation of replication and reveals that re-initiation events can occur following multiple bursts of replication. The periodic properties of these extracts are sensitive to protein synthesis inhibitors.

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Selected References

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