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. 1987 Aug;6(8):2203–2207. doi: 10.1002/j.1460-2075.1987.tb02491.x

The sexual inducer of Volvox carteri: purification, chemical characterization and identification of its gene

H Tschochner 1, F Lottspeich 1, M Sumper 1
PMCID: PMC553619  PMID: 16453787

Abstract

The sexual inducer of Volvox carteri f. nagariensis is a glycoprotein and one of the most potent biological effector molecules known. It is synthesized by sperm cells and converts asexually growing males and females to the sexual pathway. Until now, large-scale production of the inducer was made impossible by an inherent biological `switch' mechanism, the spontaneous self-induction of asexually growing males. Here we describe a method overcoming this problem for the first time. Large-scale production and purification allowed a detailed chemical characterization of the inducer with respect to partial amino acid sequences and sugar composition. Chemically synthesized oligodeoxynucleotides corresponding to derived amino acid sequences were used to screen a genomic gene bank of V. carteri HK 10. A positive clone (Ind-28) was shown to encode the inducer gene by subcloning and sequencing.

Keywords: Volvox carteri, sexual inducer, glycoprotein, `switch' mechanism

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Callahan A. M., Huskey R. J. Genetic control of sexual development in Volvox. Dev Biol. 1980 Dec;80(2):419–435. doi: 10.1016/0012-1606(80)90416-9. [DOI] [PubMed] [Google Scholar]
  2. Hattori M., Sakaki Y. Dideoxy sequencing method using denatured plasmid templates. Anal Biochem. 1986 Feb 1;152(2):232–238. doi: 10.1016/0003-2697(86)90403-3. [DOI] [PubMed] [Google Scholar]
  3. Kirk D. L., Kirk M. M. Heat shock elicits production of sexual inducer in Volvox. Science. 1986 Jan 3;231(4733):51–54. doi: 10.1126/science.3941891. [DOI] [PubMed] [Google Scholar]
  4. Kushner P. J., Blair L. C., Herskowitz I. Control of yeast cell types by mobile genes: a test. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5264–5268. doi: 10.1073/pnas.76.10.5264. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Mort A. J., Lamport D. T. Anhydrous hydrogen fluoride deglycosylates glycoproteins. Anal Biochem. 1977 Oct;82(2):289–309. doi: 10.1016/0003-2697(77)90165-8. [DOI] [PubMed] [Google Scholar]
  6. Nasmyth K. Molecular analysis of a cell lineage. Nature. 1983 Apr 21;302(5910):670–676. doi: 10.1038/302670a0. [DOI] [PubMed] [Google Scholar]
  7. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Starr R. C. Control of differentiation in Volvox. Symp Soc Dev Biol. 1970;29:59–100. doi: 10.1016/b978-0-12-395534-0.50009-1. [DOI] [PubMed] [Google Scholar]
  9. Starr R. C., Jaenicke L. Purification and characterization of the hormone initiating sexual morphogenesis in Volvox carteri f. nagariensis Iyengar. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1050–1054. doi: 10.1073/pnas.71.4.1050. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Wenzl S., Thym D., Sumper M. Development-dependent modification of the extracellular matrix by a sulphated glycoprotein in Volvox carteri. EMBO J. 1984 Apr;3(4):739–744. doi: 10.1002/j.1460-2075.1984.tb01877.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Wieland F., Heitzer R., Schaefer W. Asparaginylglucose: Novel type of carbohydrate linkage. Proc Natl Acad Sci U S A. 1983 Sep;80(18):5470–5474. doi: 10.1073/pnas.80.18.5470. [DOI] [PMC free article] [PubMed] [Google Scholar]

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