Fig. 4.
Effects of blebbistatin exposure on growth of the SPL during Phase 1. (A,B) Embryo with attached beads at HH5 and after 3 h of culture. Two representative triangles used to compute tissue strains are indicated. Analyzed beads were taken from the regions marked by red ovals. (C) Growth ratios Gx and Gy computed from strain and stress data (see supplementary Materials and Methods). Results are shown for control embryos (n=8) and blebbistatin-treated embryos (n=7) (two-way ANOVA, *P<0.01). Blebbistatin exposure slowed but did not stop growth in both the x and y directions.