Fig. 5.

Adult temperature shifts have a delayed impact on the maternal effect. (A) (simw⁵⁰¹); OreR females were raised at 25°C or 28°C, kept at the same temperature, or shifted to the reciprocal temperature on day 1 of adulthood, and then crossed to y w males. Embryos from these females were collected on days 3-12 of adulthood, allowed to develop at 25°C and hatch rates were measured at 36 h. (B) Hatch rates of embryos from (simw⁵⁰¹); OreR mothers who were treated as described in A. n=3 biological replicates; data are mean±s.e.m.. Red asterisks represent P values for comparison of 25-28°C shift (red line) versus constant 25°C (blue line). Black asterisks represent P values for comparison of 28-25°C shift (black line) versus constant 28°C (gold line). *P<0.05; **P<0.01; ***P<0.001. n=3.