Fig. 8.

Temperature-sensitive mito-nuclear incompatibility has pleiotropic effects on female fertility. At a higher temperature (28°C), the OreR nuclear genotype causes loss of GSCs, reduced egg chamber production and degeneration of egg chambers through apoptosis and autophagy, all of which are enhanced by incompatibility with simw⁵⁰¹ mitochondria. These ovarian phenotypes together contribute to greatly decreased egg production. Temperature-sensitive mito-nuclear incompatibility in (simw⁵⁰¹); OreR females caused unique nurse cell dumping and eggshell synthesis defects during later oogenesis. Below: temperature-sensitive mito-nuclear incompatibility in (simw⁵⁰¹); OreR mothers resulted in mitotic errors in their embryos, which may be caused by insufficient maternal metabolites or maternal inheritance of sub-functional mitochondria.