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. 2017 Jul 31;6:e26404. doi: 10.7554/eLife.26404

Figure 5. REMORIN C-terminal anchor defines protein mobility in the PM.

Figure 5.

( A ) Super-resolved trajectories ( trajectories > 20 points ;) of EOS- St REM1.3 WT and REM-CA mutants (K183S, K192A, K183S / K192A and K192A / K193A) visualized by high-resolution microscopy spt-PALM VAEM . 3. ( B, C ) Distribution of diffusion coefficients ( D ) represented as log (D) of the different fusion proteins and distributions of the peak D values ​​of individual cells obtained by normal fits and were plotted as log (D), bar indicates mean ± sem ( D ) Mean Square Displacement ( MSD ) over time for the global trajectories > 15 pointsof each EOS- St REM1.3 construct ( n = 13 to 51 cells over three independent experiments). ( E ) Representative trajectories of St REM1.3 WT and REM-CA mutants. Significant differences revealed by Dunn's multiple comparisons.