Figure 3. Light-mediated recruitment of Cdc24 directs bud site positioning.
(A) Domain schematic of Cdc24-ePDZ and variants thereof. (B) Polarization efficiency of photo-illuminated (red) or mock-illuminated (blue) cells. Data are averaged across multiple experiments (n experiments > = 2, N total cells > 15, for each group). Average and ± S.E.M. indicated. Polarization efficiency of Cdc24 and Cdc24ΔPB1 were statistically significant relative to Cdc24-GEF-dead light/dark, and statistically significant compared to their respective dark state controls. The difference between Cdc24 and Cdc24ΔPB1 were not statistically significant. The response to Cdc24-GEF dead was not statistically significant relative to its dark state control. p<0.05, Mann-Whitney U test. Strains used: WYK8440, WYK8437, and WYK8439. (C) Panels of representative phase and fluorescence images and kymographs showing the position of the laser target and accumulation of a Cdc42 biosensor or endogenous Bem1 in response to Cdc24 recruitment. Each image is 16.2 µm x 16.2 µm. Strains used: WYK8440 and WYK8301. (D) Accumulation kinetics for the Cdc42 biosensor or endogenous Bem1 in response to Cdc24 recruitment. Data are combined across multiple experiments (n experiments >= 2, N total cells > 15 for each condition).
DOI: http://dx.doi.org/10.7554/eLife.26722.013
Figure 3—figure supplement 1. Light-induced recruitment of Cdc24 induce precocious Cdc42 activation, but does not alter Bem1 kinetics.
