Figure 3.

NDRG1 suppresses cancer cell migration and invasion through inhibiting caveolin-1 expression: NDRG1 and cav1 overexpression results. (a) The NDRG1 overexpression and relative control HT29, SW480 and SW11116 cells were incubated with or without caveolin-1-specific overexpression transfection (cav1). Whole-cell lysates were extracted and immunoblotting was performed to assess NDRG1 and caveolin-1 expression in cells transfected with Con/Vector, NDRG1/Vector, Con/cav1 and NDRG1/cav1, respectively. ***P<0.001, relative to the respective control cells. (b) Transwell assays: cells were seeded in upper chamber of insert and the migrated/invaded cells were examined after 24~48 h. Data represent the means±s.d. from three independent experiments. Representative photos of stained cells are shown with the original magnification of × 100; scale bar, 50 μm.