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. 2017 Mar 27;36(30):4323–4335. doi: 10.1038/onc.2017.74

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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NDRG1 regulates EMT through modulating caveolin-1 expression. (a) Bright-field images were taken to show cell morphological changes after sh-NDRG1 and/or SH-caveolin-1 treatment. Scale bar, 100 μm. (b) Immunoblot results of molecular markers of EMT after sh-NDRG1 and/or SH-caveolin-1 treatment. Densitometry analysis is expressed relative to the loading control, β-actin. Results are typical of three to five experiments and the histograms represent mean±s.d. (three to five experiments). *P<0.05; **P<0.01; ***P<0.001, relative to the respective control cells. (c) Bright-field images were taken to show cell morphological changes after NDRG1 and/or caveolin-1 overexpression treatment. Scale bar, 100 μm. (d) Immunoblot results of molecular markers of EMT after NDRG1 and/or caveolin-1 overexpression treatment. Densitometry analysis is expressed relative to the loading control, β-actin. Results are typical of three to five experiments and the histograms represent mean±s.d. (three to five experiments). **P<0.01, ***P<0.001, relative to the respective control cells.