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. 1987 Nov;6(11):3209–3212. doi: 10.1002/j.1460-2075.1987.tb02637.x

Biological function of `pathogenesis-related' proteins: four PR proteins of tobacco have 1,3-β-glucanase activity

Serge Kauffmann 1, Michel Legrand 1, Pierrette Geoffroy 1, Bernard Fritig 1
PMCID: PMC553771  PMID: 16453802

Abstract

Three of the ten acidic `pathogenesis-related' (PR) proteins known to accumulate in Nicotiana tabacum cv Samsun NN reacting hypersensitively to tobacco mosaic virus, namely −O, −N and −2, have been shown to have 1,3-β-glucanase (EC 3.2.1.39) activity. By using sera raised against each protein purified to homogeneity close serological relationships have been demonstrated between the three proteins. The same specific sera cross-reacted with a basic protein which is also a 1,3-β-glucanase induced by virus infection and which can be considered as a new basic pathogenesis-related protein of tobacco. Protein PR-O and the basic 1,3-β-glucanase display about the same specific enzymatic activity, i.e. 50-fold and 250-fold higher than specific activities of proteins PR-N and -2 respectively.

Keywords: 1,3-β-glucanases; purification; specific activities; antibodies; serological relationships

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Selected References

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