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. 1987 Nov;6(11):3291–3297. doi: 10.1002/j.1460-2075.1987.tb02648.x

Construction of a detailed molecular map of the mouse X chromosome by microcloning and interspecific crosses.

N Brockdorff 1, E M Fisher 1, J S Cavanna 1, M F Lyon 1, S D Brown 1
PMCID: PMC553782  PMID: 2448136

Abstract

A large number of microclones obtained by microdissection of the mouse X chromosome have been mapped using an interspecific Mus domesticus/Mus spretus cross. Clones displaying close linkage to a number of loci of known phenotype but unknown gene product, such as mdx (X-linked muscular dystrophy), have been obtained. Over a central 30 cM span of the mouse X chromosome, 17 clones have been mapped and ordered at a sufficient density to contemplate the complete physical mapping of this region that will aid in the isolation of a number of unidentified genes. Some of the mapped microclones detect moderately repetitive sequences that were clustered in several discrete regions of the mouse X chromosome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amar L. C., Arnaud D., Cambrou J., Guenet J. L., Avner P. R. Mapping of the mouse X chromosome using random genomic probes and an interspecific mouse cross. EMBO J. 1985 Dec 30;4(13B):3695–3700. doi: 10.1002/j.1460-2075.1985.tb04137.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barlow D. P., Bućan M., Lehrach H., Hogan B. L., Gough N. M. Close genetic and physical linkage between the murine haemopoietic growth factor genes GM-CSF and Multi-CSF (IL3). EMBO J. 1987 Mar;6(3):617–623. doi: 10.1002/j.1460-2075.1987.tb04799.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bates G. P., Wainwright B. J., Williamson R., Brown S. D. Microdissection of and microcloning from the short arm of human chromosome 2. Mol Cell Biol. 1986 Nov;6(11):3826–3830. doi: 10.1128/mcb.6.11.3826. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brockdorff N., Cross G. S., Cavanna J. S., Fisher E. M., Lyon M. F., Davies K. E., Brown S. D. The mapping of a cDNA from the human X-linked Duchenne muscular dystrophy gene to the mouse X chromosome. Nature. 1987 Jul 9;328(6126):166–168. doi: 10.1038/328166a0. [DOI] [PubMed] [Google Scholar]
  5. Bulfield G., Siller W. G., Wight P. A., Moore K. J. X chromosome-linked muscular dystrophy (mdx) in the mouse. Proc Natl Acad Sci U S A. 1984 Feb;81(4):1189–1192. doi: 10.1073/pnas.81.4.1189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Burmeister M., Lehrach H. Long-range restriction map around the Duchenne muscular dystrophy gene. Nature. 1986 Dec 11;324(6097):582–585. doi: 10.1038/324582a0. [DOI] [PubMed] [Google Scholar]
  7. Carle G. F., Frank M., Olson M. V. Electrophoretic separations of large DNA molecules by periodic inversion of the electric field. Science. 1986 Apr 4;232(4746):65–68. doi: 10.1126/science.3952500. [DOI] [PubMed] [Google Scholar]
  8. Feinberg A. P., Vogelstein B. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem. 1984 Feb;137(1):266–267. doi: 10.1016/0003-2697(84)90381-6. [DOI] [PubMed] [Google Scholar]
  9. Fisher E. M., Cavanna J. S., Brown S. D. Microdissection and microcloning of the mouse X chromosome. Proc Natl Acad Sci U S A. 1985 Sep;82(17):5846–5849. doi: 10.1073/pnas.82.17.5846. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Friend S. H., Bernards R., Rogelj S., Weinberg R. A., Rapaport J. M., Albert D. M., Dryja T. P. A human DNA segment with properties of the gene that predisposes to retinoblastoma and osteosarcoma. Nature. 1986 Oct 16;323(6089):643–646. doi: 10.1038/323643a0. [DOI] [PubMed] [Google Scholar]
  11. Grosschedl R., Weaver D., Baltimore D., Costantini F. Introduction of a mu immunoglobulin gene into the mouse germ line: specific expression in lymphoid cells and synthesis of functional antibody. Cell. 1984 Oct;38(3):647–658. doi: 10.1016/0092-8674(84)90259-9. [DOI] [PubMed] [Google Scholar]
  12. Lyon M. F., Zenthon J., Evans E. P., Burtenshaw M. D., Wareham K. A., Williams E. D. Lack of inactivation of a mouse X-linked gene physically separated from the inactivation centre. J Embryol Exp Morphol. 1986 Sep;97:75–85. [PubMed] [Google Scholar]
  13. Monaco A. P., Neve R. L., Colletti-Feener C., Bertelson C. J., Kurnit D. M., Kunkel L. M. Isolation of candidate cDNAs for portions of the Duchenne muscular dystrophy gene. Nature. 1986 Oct 16;323(6089):646–650. doi: 10.1038/323646a0. [DOI] [PubMed] [Google Scholar]
  14. Poustka A., Pohl T. M., Barlow D. P., Frischauf A. M., Lehrach H. Construction and use of human chromosome jumping libraries from NotI-digested DNA. Nature. 1987 Jan 22;325(6102):353–355. doi: 10.1038/325353a0. [DOI] [PubMed] [Google Scholar]
  15. Robert B., Barton P., Minty A., Daubas P., Weydert A., Bonhomme F., Catalan J., Chazottes D., Guénet J. L., Buckingham M. Investigation of genetic linkage between myosin and actin genes using an interspecific mouse back-cross. Nature. 1985 Mar 14;314(6007):181–183. doi: 10.1038/314181a0. [DOI] [PubMed] [Google Scholar]
  16. Röhme D., Fox H., Herrmann B., Frischauf A. M., Edström J. E., Mains P., Silver L. M., Lehrach H. Molecular clones of the mouse t complex derived from microdissected metaphase chromosomes. Cell. 1984 Mar;36(3):783–788. doi: 10.1016/0092-8674(84)90358-1. [DOI] [PubMed] [Google Scholar]
  17. Scalenghe F., Turco E., Edström J. E., Pirrotta V., Melli M. Microdissection and cloning of DNA from a specific region of Drosophila melanogaster polytene chromosomes. Chromosoma. 1981;82(2):205–216. doi: 10.1007/BF00286105. [DOI] [PubMed] [Google Scholar]
  18. Schwartz D. C., Cantor C. R. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell. 1984 May;37(1):67–75. doi: 10.1016/0092-8674(84)90301-5. [DOI] [PubMed] [Google Scholar]
  19. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  20. Steinmetz M., Stephan D., Fischer Lindahl K. Gene organization and recombinational hotspots in the murine major histocompatibility complex. Cell. 1986 Mar 28;44(6):895–904. doi: 10.1016/0092-8674(86)90012-7. [DOI] [PubMed] [Google Scholar]

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