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. 1987 Nov;6(11):3313–3316. doi: 10.1002/j.1460-2075.1987.tb02651.x

Molecular cloning of two CD7 (T-cell leukemia antigen) cDNAs by a COS cell expression system.

A Aruffo 1, B Seed 1
PMCID: PMC553785  PMID: 3501369

Abstract

The human CD7 antigen (gp40) is a cell surface glycoprotein found on thymocytes and mature T-cells. It is one of the earliest antigens to appear on cells of the T-lymphocyte lineage, and the most reliable clinical marker of T-cell acute lymphocytic leukemia. This report describes the isolation and nucleotide sequence of a full length CD7 cDNA, and of a cDNA for an unusual intron-bearing precursor. The DNA sequence of the clone predicts a highly glycosylated membrane protein with homology to members of the immunoglobulin superfamily, and no relationship to known oncogenes. Over-expression of CD7 RNA was observed in only one T-cell tumor line, and genomic DNA rearrangement was not observed in any lines. Prompted by a recent suggestion that CD7 plays a role in IgM binding, COS cells expressing CD7 were tested and found not to bind IgM or IgM immune complexes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ercolani L., Novogrodsky A., Suthanthiran M., Rubin A. L., Stenzel K. H. Expression of Fc mu receptors by human T lymphocytes: effects of enzymes, metabolic inhibitors, and X-irradiation. J Immunol. 1981 Nov;127(5):2044–2051. [PubMed] [Google Scholar]
  2. Hardin J. A., Nakaoka K. K., Carboni J. M. IgM receptors on human lymphocytes: detection by direct binding. Proc Natl Acad Sci U S A. 1979 Feb;76(2):912–914. doi: 10.1073/pnas.76.2.912. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Haynes B. F., Eisenbarth G. S., Fauci A. S. Human lymphocyte antigens: production of a monoclonal antibody that defines functional thymus-derived lymphocyte subsets. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5829–5833. doi: 10.1073/pnas.76.11.5829. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  5. Johnson P., Williams A. F. Striking similarities between antigen receptor J pieces and sequence in the second chain of the murine CD8 antigen. Nature. 1986 Sep 4;323(6083):74–76. doi: 10.1038/323074a0. [DOI] [PubMed] [Google Scholar]
  6. Kaufman J. F., Krangel M. S., Strominger J. L. Cysteines in the transmembrane region of major histocompatibility complex antigens are fatty acylated via thioester bonds. J Biol Chem. 1984 Jun 10;259(11):7230–7238. [PubMed] [Google Scholar]
  7. Lemke G., Axel R. Isolation and sequence of a cDNA encoding the major structural protein of peripheral myelin. Cell. 1985 Mar;40(3):501–508. doi: 10.1016/0092-8674(85)90198-9. [DOI] [PubMed] [Google Scholar]
  8. Link M., Warnke R., Finlay J., Amylon M., Miller R., Dilley J., Levy R. A single monoclonal antibody identifies T-cell lineage of childhood lymphoid malignancies. Blood. 1983 Oct;62(4):722–728. [PubMed] [Google Scholar]
  9. McConnell I., Hurd C. M. Lymphocyte receptors. II. Receptors for rabbit IgM on human T lymphocytes. Immunology. 1976 Jun;30(6):835–839. [PMC free article] [PubMed] [Google Scholar]
  10. Moretta L., Ferrarini M., Durante M. L., Mingari M. C. Expression of a receptor for IgM by human T cells in vitro. Eur J Immunol. 1975 Aug;5(8):565–569. doi: 10.1002/eji.1830050812. [DOI] [PubMed] [Google Scholar]
  11. Morishima Y., Kobayashi M., Yang S. Y., Collins N. H., Hoffmann M. K., Dupont B. Functionally different T lymphocyte subpopulations determined by their sensitivity to complement-dependent cell lysis with the monoclonal antibody 4A. J Immunol. 1982 Sep;129(3):1091–1098. [PubMed] [Google Scholar]
  12. Mount S. M. A catalogue of splice junction sequences. Nucleic Acids Res. 1982 Jan 22;10(2):459–472. doi: 10.1093/nar/10.2.459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ramakrishnan S., Uckun F. M., Houston L. L. Anti-T cell immunotoxins containing pokeweed anti-viral protein: potential purging agents for human autologous bone marrow transplantation. J Immunol. 1985 Nov;135(5):3616–3622. [PubMed] [Google Scholar]
  14. Rose J. K., Adams G. A., Gallione C. J. The presence of cysteine in the cytoplasmic domain of the vesicular stomatitis virus glycoprotein is required for palmitate addition. Proc Natl Acad Sci U S A. 1984 Apr;81(7):2050–2054. doi: 10.1073/pnas.81.7.2050. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Seed B., Aruffo A. Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure. Proc Natl Acad Sci U S A. 1987 May;84(10):3365–3369. doi: 10.1073/pnas.84.10.3365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sutherland D. R., Rudd C. E., Greaves M. F. Isolation and characterization of a human T lymphocyte-associated glycoprotein (gp40). J Immunol. 1984 Jul;133(1):327–333. [PubMed] [Google Scholar]
  17. Thurlow P. J., Lovering K. E., McKenzie I. F. A monoclonal antibody detecting a new human T cell antigen, HuLy-m2. Transplantation. 1984 Aug;38(2):143–147. doi: 10.1097/00007890-198408000-00010. [DOI] [PubMed] [Google Scholar]
  18. Tse A. G., Barclay A. N., Watts A., Williams A. F. A glycophospholipid tail at the carboxyl terminus of the Thy-1 glycoprotein of neurons and thymocytes. Science. 1985 Nov 29;230(4729):1003–1008. doi: 10.1126/science.2865810. [DOI] [PubMed] [Google Scholar]
  19. Williams A. F., Gagnon J. Neuronal cell Thy-1 glycoprotein: homology with immunoglobulin. Science. 1982 May 14;216(4547):696–703. doi: 10.1126/science.6177036. [DOI] [PubMed] [Google Scholar]
  20. van Dongen J. J., Hooijkaas H., Comans-Bitter M., Hählen K., de Klein A., van Zanen G. E., van't Veer M. B., Abels J., Benner R. Human bone marrow cells positive for terminal deoxynucleotidyl transferase (TdT), HLA-DR, and a T cell marker may represent prothymocytes. J Immunol. 1985 Nov;135(5):3144–3150. [PubMed] [Google Scholar]
  21. von Heijne G. A new method for predicting signal sequence cleavage sites. Nucleic Acids Res. 1986 Jun 11;14(11):4683–4690. doi: 10.1093/nar/14.11.4683. [DOI] [PMC free article] [PubMed] [Google Scholar]

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