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. 1987 Nov;6(11):3317–3324. doi: 10.1002/j.1460-2075.1987.tb02652.x

Purification of KBF1, a common factor binding to both H-2 and beta 2-microglobulin enhancers.

O Yano 1, J Kanellopoulos 1, M Kieran 1, O Le Bail 1, A Israël 1, P Kourilsky 1
PMCID: PMC553786  PMID: 3322806

Abstract

An enhancer binding factor, designated KBF1, has been purified from the nuclear extract of mouse BW5147 thymoma cells by five column chromatography steps including a sequence-specific DNA affinity column. Gel retardation and footprint analysis have shown that purified KBF1 has a binding activity specific for both H-2 and beta 2-microglobulin enhancer sequences. After SDS-polyacrylamide gel electrophoresis of the most purified preparation a 48-kd protein showed, after elution and renaturation, a binding activity to both enhancer sequences. These findings suggest that the expression of both H-2 and beta 2-microglobulin genes utilizes a common regulatory mechanism.

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