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. 1987 Nov;6(11):3539–3542. doi: 10.1002/j.1460-2075.1987.tb02680.x

gal4 transcription activator protein of yeast can function as a repressor in Escherichia coli.

N Paulmier 1, M Yaniv 1, B von Wilcken-Bergmann 1, B Müller-Hill 1
PMCID: PMC553814  PMID: 3123219

Abstract

The chromosomal lac operator of Escherichia coli was replaced by a 22 bp oligonucleotide containing the binding site of the yeast gal4 protein. Induction of gal4 protein synthesis in these bacteria repressed beta-galactosidase synthesis at least 30-fold. These results show that it is possible to detect in bacteria with a simple assay the DNA binding activity of a eukaryotic protein with a defined sequence specificity. This opens new avenues for the isolation in E. coli of mutants of DNA binding proteins unable to bind to their DNA targets, and for direct cloning in bacteria of cDNA coding for DNA binding proteins with defined sequence specificity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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