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. 1987 Dec 20;6(13):3881–3889. doi: 10.1002/j.1460-2075.1987.tb02728.x

Studies on the introduction and mobility of the maize Activator element in Arabidopsis thaliana and Daucus carota.

M A Van Sluys 1, J Tempé 1, N Fedoroff 1
PMCID: PMC553865  PMID: 2832144

Abstract

We have co-transformed carrot (Daucus carota) and Arabidopsis thaliana with an Agrobacterium tumefaciens non-tumorigenic T-DNA carrying the maize transposable element Activator (Ac) and an Agrobacterium rhizogenes Ri T-DNA. We present evidence that the Ac element transposes in transformed root or root-derived callus cultures of both species. We show that fertile plants can be regenerated from transformed, root-derived callus cultures of Arabidopsis, demonstrating the utility of the Ri plasmid for introducing the maize Ac element into plants. We also present evidence that Ac elements that excise from the transforming T-DNA early after transformation continue to be mobile in carrot root cultures.

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Selected References

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  1. Baker B., Coupland G., Fedoroff N., Starlinger P., Schell J. Phenotypic assay for excision of the maize controlling element Ac in tobacco. EMBO J. 1987 Jun;6(6):1547–1554. doi: 10.1002/j.1460-2075.1987.tb02399.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baker B., Schell J., Lörz H., Fedoroff N. Transposition of the maize controlling element "Activator" in tobacco. Proc Natl Acad Sci U S A. 1986 Jul;83(13):4844–4848. doi: 10.1073/pnas.83.13.4844. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cone K. C., Burr F. A., Burr B. Molecular analysis of the maize anthocyanin regulatory locus C1. Proc Natl Acad Sci U S A. 1986 Dec;83(24):9631–9635. doi: 10.1073/pnas.83.24.9631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Costantino P., Spanò L., Pomponi M., Benvenuto E., Ancora G. The T-DNA of Agrobacterium rhizogenes is transmitted through meiosis to the progeny of hairy root plants. J Mol Appl Genet. 1984;2(5):465–470. [PubMed] [Google Scholar]
  5. Czernilofsky A. P., Hain R., Baker B., Wirtz U. Studies of the structure and functional organization of foreign DNA integrated into the genome of Nicotiana tabacum. DNA. 1986 Dec;5(6):473–482. doi: 10.1089/dna.1.1986.5.473. [DOI] [PubMed] [Google Scholar]
  6. De Paolis A., Mauro M. L., Pomponi M., Cardarelli M., Spanò L., Costantino P. Localization of agropine-synthesizing functions in the TR region of the root-inducing plasmid of Agrobacterium rhizogenes 1855. Plasmid. 1985 Jan;13(1):1–7. doi: 10.1016/0147-619x(85)90050-2. [DOI] [PubMed] [Google Scholar]
  7. Döring H. P., Starlinger P. Molecular genetics of transposable elements in plants. Annu Rev Genet. 1986;20:175–200. doi: 10.1146/annurev.ge.20.120186.001135. [DOI] [PubMed] [Google Scholar]
  8. Fedoroff N. V., Furtek D. B., Nelson O. E. Cloning of the bronze locus in maize by a simple and generalizable procedure using the transposable controlling element Activator (Ac). Proc Natl Acad Sci U S A. 1984 Jun;81(12):3825–3829. doi: 10.1073/pnas.81.12.3825. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Herrera-Estrella L., Block M. D., Messens E., Hernalsteens J. P., Montagu M. V., Schell J. Chimeric genes as dominant selectable markers in plant cells. EMBO J. 1983;2(6):987–995. doi: 10.1002/j.1460-2075.1983.tb01532.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jouanin L. Restriction map of an agropine-type Ri plasmid and its homologies with Ti plasmids. Plasmid. 1984 Sep;12(2):91–102. doi: 10.1016/0147-619x(84)90055-6. [DOI] [PubMed] [Google Scholar]
  11. Kessler C., Höltke H. J. Specificity of restriction endonucleases and methylases--a review. Gene. 1986;47(1):1–153. doi: 10.1016/0378-1119(86)90245-3. [DOI] [PubMed] [Google Scholar]
  12. Kunze R., Stochaj U., Laufs J., Starlinger P. Transcription of transposable element Activator (Ac) of Zea mays L. EMBO J. 1987 Jun;6(6):1555–1563. doi: 10.1002/j.1460-2075.1987.tb02400.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McCLINTOCK B. Chromosome organization and genic expression. Cold Spring Harb Symp Quant Biol. 1951;16:13–47. doi: 10.1101/sqb.1951.016.01.004. [DOI] [PubMed] [Google Scholar]
  14. Meyerowitz E. M., Pruitt R. E. Arabidopsis thaliana and Plant Molecular Genetics. Science. 1985 Sep 20;229(4719):1214–1218. doi: 10.1126/science.229.4719.1214. [DOI] [PubMed] [Google Scholar]
  15. O'Reilly C., Shepherd N. S., Pereira A., Schwarz-Sommer Z., Bertram I., Robertson D. S., Peterson P. A., Saedler H. Molecular cloning of the a1 locus of Zea mays using the transposable elements En and Mu1. EMBO J. 1985 Apr;4(4):877–882. doi: 10.1002/j.1460-2075.1985.tb03713.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pohlman R. F., Fedoroff N. V., Messing J. The nucleotide sequence of the maize controlling element Activator. Cell. 1984 Jun;37(2):635–643. doi: 10.1016/0092-8674(84)90395-7. [DOI] [PubMed] [Google Scholar]
  17. Rosen E., Sivertsen A., Firtel R. A. An unusual transposon encoding heat shock inducible and developmentally regulated transcripts in Dictyostelium. Cell. 1983 Nov;35(1):243–251. doi: 10.1016/0092-8674(83)90227-1. [DOI] [PubMed] [Google Scholar]
  18. Rédei G. P. Arabidopsis as a genetic tool. Annu Rev Genet. 1975;9:111–127. doi: 10.1146/annurev.ge.09.120175.000551. [DOI] [PubMed] [Google Scholar]
  19. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sutton W. D., Gerlach W. L., Peacock W. J., Schwartz D. Molecular analysis of ds controlling element mutations at the adh1 locus of maize. Science. 1984 Mar 23;223(4642):1265–1268. doi: 10.1126/science.223.4642.1265. [DOI] [PubMed] [Google Scholar]
  21. Tepfer D. Transformation of several species of higher plants by Agrobacterium rhizogenes: sexual transmission of the transformed genotype and phenotype. Cell. 1984 Jul;37(3):959–967. doi: 10.1016/0092-8674(84)90430-6. [DOI] [PubMed] [Google Scholar]

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