Abstract
Enhancer of split [E(spl)], one of the neurogenic loci of Drosophila, is located in bands 96F8-13. One hundred and fifty kilobases of genomic DNA, spanning the E(spl) locus, were cloned by chromosomal walking. DNA heterogeneities associated with eleven E(spl) mutations, including three Pr alleles, were mapped to a region of 36 kb, and an additional one outside of this region. One of these mutations is a deletion of ˜34 kb that causes severe neural hyperplasia of homozygous embryos with complete penetrance. Mutations associated with DNA polymorphisms mapping within smaller regions do not lead to a fully penetrant neurogenic phenotype. The 36-kb region encodes 11 major transcripts, which exhibit distinct temporal and/or spatial patterns of expression. The expression of one of these transcripts is modified in two different mutants. In addition, one of the mutants [E(spl)D] shows another transcript, which is not present in the wild-type and co-exists with the remaining transcripts. We suggest that more than one of the 11 transcripts are necessary for a normal function of the E(spl) locus. The spatial distribution of four of these RNAs, which exhibit almost identical patterns of expression, strongly suggests that the encoded proteins are required for the process of segregation of neural and epidermal lineages.
Keywords: Drosophila, neurogenesis, Enhancer of split, organization, expression
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- Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Campuzano S., Carramolino L., Cabrera C. V., Ruíz-Gómez M., Villares R., Boronat A., Modolell J. Molecular genetics of the achaete-scute gene complex of D. melanogaster. Cell. 1985 Feb;40(2):327–338. doi: 10.1016/0092-8674(85)90147-3. [DOI] [PubMed] [Google Scholar]
- Doe C. Q., Goodman C. S. Early events in insect neurogenesis. I. Development and segmental differences in the pattern of neuronal precursor cells. Dev Biol. 1985 Sep;111(1):193–205. doi: 10.1016/0012-1606(85)90445-2. [DOI] [PubMed] [Google Scholar]
- Holmes D. S., Quigley M. A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem. 1981 Jun;114(1):193–197. doi: 10.1016/0003-2697(81)90473-5. [DOI] [PubMed] [Google Scholar]
- Kidd S., Kelley M. R., Young M. W. Sequence of the notch locus of Drosophila melanogaster: relationship of the encoded protein to mammalian clotting and growth factors. Mol Cell Biol. 1986 Sep;6(9):3094–3108. doi: 10.1128/mcb.6.9.3094. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Knust E., Bremer K. A., Vässin H., Ziemer A., Tepass U., Campos-Ortega J. A. The enhancer of split locus and neurogenesis in Drosophila melanogaster. Dev Biol. 1987 Jul;122(1):262–273. doi: 10.1016/0012-1606(87)90351-4. [DOI] [PubMed] [Google Scholar]
- Knust E., Dietrich U., Tepass U., Bremer K. A., Weigel D., Vässin H., Campos-Ortega J. A. EGF homologous sequences encoded in the genome of Drosophila melanogaster, and their relation to neurogenic genes. EMBO J. 1987 Mar;6(3):761–766. doi: 10.1002/j.1460-2075.1987.tb04818.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kuner J. M., Nakanishi M., Ali Z., Drees B., Gustavson E., Theis J., Kauvar L., Kornberg T., O'Farrell P. H. Molecular cloning of engrailed: a gene involved in the development of pattern in Drosophila melanogaster. Cell. 1985 Aug;42(1):309–316. doi: 10.1016/s0092-8674(85)80126-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pirrotta V., Hadfield C., Pretorius G. H. Microdissection and cloning of the white locus and the 3B1-3C2 region of the Drosophila X chromosome. EMBO J. 1983;2(6):927–934. doi: 10.1002/j.1460-2075.1983.tb01523.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Poole S. J., Kauvar L. M., Drees B., Kornberg T. The engrailed locus of Drosophila: structural analysis of an embryonic transcript. Cell. 1985 Jan;40(1):37–43. doi: 10.1016/0092-8674(85)90306-x. [DOI] [PubMed] [Google Scholar]
- Poulson D. F. Chromosomal Deficiencies and the Embryonic Development of Drosophila Melanogaster. Proc Natl Acad Sci U S A. 1937 Mar;23(3):133–137. doi: 10.1073/pnas.23.3.133. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reed K. C., Mann D. A. Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Res. 1985 Oct 25;13(20):7207–7221. doi: 10.1093/nar/13.20.7207. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schultz J. Variegation in Drosophila and the Inert Chromosome Regions. Proc Natl Acad Sci U S A. 1936 Jan;22(1):27–33. doi: 10.1073/pnas.22.1.27. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tartof K. D., Hobbs C., Jones M. A structural basis for variegating position effects. Cell. 1984 Jul;37(3):869–878. doi: 10.1016/0092-8674(84)90422-7. [DOI] [PubMed] [Google Scholar]
- Technau G. M., Campos-Ortega J. A. Cell autonomy of expression of neurogenic genes of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1987 Jul;84(13):4500–4504. doi: 10.1073/pnas.84.13.4500. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vässin H., Bremer K. A., Knust E., Campos-Ortega J. A. The neurogenic gene Delta of Drosophila melanogaster is expressed in neurogenic territories and encodes a putative transmembrane protein with EGF-like repeats. EMBO J. 1987 Nov;6(11):3431–3440. doi: 10.1002/j.1460-2075.1987.tb02666.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vässin H., Vielmetter J., Campos-Ortega J. A. Genetic interactions in early neurogenesis of Drosophila melanogaster. J Neurogenet. 1985 Nov;2(5):291–308. doi: 10.3109/01677068509102325. [DOI] [PubMed] [Google Scholar]
- Welshons W. J. Analysis of a gene in drosophila. Science. 1965 Nov 26;150(3700):1122–1129. doi: 10.1126/science.150.3700.1122. [DOI] [PubMed] [Google Scholar]
- Wharton K. A., Johansen K. M., Xu T., Artavanis-Tsakonas S. Nucleotide sequence from the neurogenic locus notch implies a gene product that shares homology with proteins containing EGF-like repeats. Cell. 1985 Dec;43(3 Pt 2):567–581. doi: 10.1016/0092-8674(85)90229-6. [DOI] [PubMed] [Google Scholar]
- Wright T. R. The genetics of embryogenesis in Drosophila. Adv Genet. 1970;15:261–395. doi: 10.1016/s0065-2660(08)60075-9. [DOI] [PubMed] [Google Scholar]