In the original article, there was a mistake in Table 2. Twenty of the 28 genes detected in the farmed fish intestinal contents as published. In the Table 2, the numbers of average relative abundance of the 20 genes to the 16S rRNA gene were not correct. The corrected numbers of the gene abundances appears below. We apologize for this error and the error does not change the scientific conclusions of the article in any way.
Table 2.
Classification of the antibiotics the genes confer resistance to | qPCR assay | Average relative abundance to the 16S rRNA gene | |
---|---|---|---|
Fish intestinal contents (sampled in 2014) | Fish farm sediments (sampled in 2006–2012) | ||
Aminoglycoside | aadA1 | 2 × 10−2 | a3 × 10−4 |
Aminoglycoside | aadA2-01 | 2 × 10−2 | a2 × 10−4 |
Aminoglycoside | aadA2-02 | 2 × 10−2 | a4 × 10−4 |
Aminoglycoside | aadA2-03 | 4 × 10−2 | a8 × 10−4 |
Trimethoprim | dfrA1 | 6 × 10−2 | a3 × 10−3 |
Trimethoprim | dfrA1-02 | 4 × 10−2 | b1 × 10−3 |
Class 1 integron | intI1 | 6 × 10−2 | b3 × 10−3 |
Other (Antiseptic) | qacEΔ1-01 | 1 × 10−2 | a5 × 10−3 |
Other (Antiseptic) | qacEΔ1-02 | 1 × 10−1 | a3 × 10−3 |
Sulfonamide | sul1 | 7 × 10−2 | b4 × 10−3 |
Tetracycline | tet(32) | 3 × 10−2 | a1 × 10−3 |
Tetracycline | tetM-01 | 1 × 10−1 | a3 × 10−3 |
Tetracycline | tetM-02 | 9 × 10−2 | a2 × 10−3 |
Tetracycline | tetM-03 | 4 × 10−2 | c1 × 10−3 |
Tetracycline | tetO-01 | 2 × 10−2 | a2 × 10−3 |
Tetracycline | tetW-01 | 4 × 10−2 | a4 × 10−4 |
Transposon | tnpA-01 | 3 × 10−2 | a6 × 10−4 |
Transposon | tnpA-04 | 4 × 10−2 | a2 × 10−4 |
Transposon | tnpA-06 | 5 × 10−1 | a1 × 10−3 |
Transposon | tnpA-07 | 4 × 10−2 | a4 × 10−3 |
Muziasari et al., 2016.
Muziasari et al., 2014.
Tamminen et al., 2011.
b,cThe quantification of the genes used standard qPCR.
The 20 genes were the same genes found to be enriched in the sediments below fish farms in the Northern Baltic Sea, Finland. The table also shows the average relative abundance of the genes to the 16S rRNA gene in the intestinal contents and in the farm sediments. The gene assays of the qPCR array grouped by classification of the antibiotics the genes confer resistance to, class 1 integron and transposon.
Conflict of interest statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.